Kinase Wee1 fusion protein compositions, nucleotide sequences, expression systems, and methods of use
AssigneeThe Scripps Research Institute
InventorsPaul Russell, Michael N. Boddy, Beth Furnari
Life of this patent
Estimating the term…
Abstract
The invention describes recombinant fusion proteins containing Weel protein sequences involved in checkpoint regulation of cell cycle, nucleic acid molecules that encode the fusion protein, and methods using the proteins for screening for compounds which modulate Weel or Cds1 function.
Claims48
An isolated nucleotide sequence that encodes a truncated Weel protein comprising amino acid residues 11-72 of the native Weel protein, and homologs thereof.
An expression vector comprising a nucleotide sequence that encodes a truncated Weel protein comprising amino acid residues 11-72 of the native Weel protein, wherein said vector is capable of expressing said truncated Weel protein encoded by said nucleotide sequence.
A recombinant truncated Weel protein comprising amino acid residues 11-72 of the native Weel protein.
A method for identifying a compound which modulates Cds1 kinase activity comprising the steps of:
- a) combining:
- i) a test compound;
- ii) a truncated Weel protein comprising amino acid residues 11-72 of the native Weel protein, or a homolog thereof;
- and
- iii) a composition comprising Cds1 protein;
- b) maintaining the combination of step (a) under conditions sufficient for Cds1 to phosphorylate said truncated Weel protein;
- and
- c) determining the amount of phosphorylated Weel protein whereby the amount of phosphorylated Weel indicates whether said test compound modulates the activity of Cds1.
A method for identifying a compound which modulates kinase Weel activity comprising the steps of:
- a) combining:
- i) a test compound;
- ii) a truncated Weel protein comprising amino acid residues 11-72 of the native Weel protein, or a homolog thereof;
- and
- iii) a reagent for Weel;
- b) maintaining the combination of step (a) under conditions suitable for Weel to react with said reagent;
- and
- c) determining the amount Weel reacts with said reagent whereby the amount of reaction indicates whether said test compound modulates the activity of Weel.
Dependencies are inferred from the claim text.