Rhodococcus gene encoding aldoxime dehydratase
AssigneeE. I. Du Pont de Nemours and Company
InventorsMichael G. Bramucci, Vasantha Nagarajan, Mario W. Chen
Life of this patent
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Abstract
A gene has been isolated from a Rhodococcus sp. encoding an aldoxime dehydratase enzyme useful for the conversion of aldoxime substrates to nitrilases and other downstream intermediates. The gene has been cloned into a recombinant host and expressed.
Claims18
An isolated nucleic acid molecule encoding an aldoxime dehydratase, selected from the group consisting of:
- (a) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO:2;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1× SSC, 0.1% SDS, 65° C. and washed with 2× SSC, 0.1% SDS followed by 0.1× SSC, 0.1% SDS;
- or an isolated nucleic acid fragment that is complementary to (a) or (b).
An isolated nucleic acid molecule as set forth in SEQ ID NO:
- 1.
A polypeptide having the amino acid sequence as set forth in SEQ ID NO:2.
An isolated nucleic acid molecule comprising a first nucleotide sequence encoding a polypeptide of at least 353 amino acids that has at least 60% identity based on the Smith-Waterman method of alignment when compared to a polypeptide have the sequence as set forth in SEQ ID NO:2 or a second nucleotide sequence comprising the complement of the first nucleotide sequence, wherein said enzyme has aldoxime dehydratase activity.
Dependencies are inferred from the claim text.