Structure for presenting desired peptide sequences
OwnerErwin Houtzager, Irma Maria Caecilia Vijn +1inventors
InventorsErwin Houtzager, Irma Maria Caecilia Vijn, Peter Christiaan Sijmons
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Abstract
Means and methods for generating binding peptide associated with a suitable core region are disclosed, the resulting proteinaceous molecule and uses thereof. A solution to the problems associated with the use of binding molecules over their entire range of use. Binding molecules can be designed to accommodate extreme conditions of use such as extreme temperatures or pH. Alternatively, binding molecules can be designed to respond to very subtle changes in the environment.
Claims55
An isolated proteinaceous molecule comprising:
- a binding peptide;
- and
- a core comprising a β-barrel of at least 4 strands, wherein said β-barrel comprises at least two β-sheets, wherein each of said at least two β-sheets comprises two of said at least 4 strands,
- wherein said binding peptide connects two strands of said β-barrel and is outside a natural context of said binding peptide.
A process for producing a nucleic acid encoding a proteinaceous molecule capable of displaying at least one desired peptide sequence, said process comprising:
- providing a nucleic acid sequence encoding at least a first and second structural region separated by a second nucleic acid sequence encoding said at least one desired peptide sequence or a region where said second nucleic acid sequence can be inserted;
- and
- mutating said nucleic acid sequence encoding said first and second structural regions to obtain the nucleic acid encoding said proteinaceous molecule capable of displaying at least one desired peptide sequence.
A process for producing a library including artificial binding peptides, said process comprising:
- providing at least one nucleic acid template, wherein each of said at least one nucleic acid templates encode different specific binding peptides;
- producing a collection of nucleic acid derivatives by mutating said at least one nucleic acid templates;
- and
- providing at least a portion of said collection to a peptide synthesis system to produce said library.
A nucleic acid produced by a process, said process comprising:
- providing a nucleic acid sequence encoding at least a first and second structural region separated by a second nucleic acid sequence encoding said at least one desired peptide sequence or a region where said second nucleic acid sequence can be inserted;
- and
- mutating said nucleic acid sequence encoding said first and second structural regions to obtain the second nucleic acid encoding said proteinaceous molecule capable of displaying at least one desired peptide sequence.
A proteinaceous molecule comprising a peptide sequence selected from the group consisting of the peptide sequences illustrated in Table 2, 3, 10, 13 and 16.
Dependencies are inferred from the claim text.