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US 2003/0215847 A1Published application
Human cartilage glycoprotein
AssigneeSmithKline Beecham Corporation
InventorsRobert B. Kirkpatrick, Martin Rosenberg
Life of this patent
Estimating the term…
Abstract
Nucleic acid sequences for HC gp-39L are provided. Methods of detecting altered expression of tissue remodeling proteins and diagnosing tissue remodeling disorders are also provided.
Claims7
A nucleic acid sequence encoding HC gp-39L.
A nucleic acid sequence comprising the sequence of SEQ ID NO:
- 1.
A method of diagnosing a tissue remodeling disorder related to expression of a mutated HC gp-39L protein in a host comprising:
- (a) synthesizing PCR primers complementary to a nucleic acid encoding a normal HC gp-39L protein;
- (b) obtaining nucleic acids from cells of a host;
- (c) amplifying the obtained nucleic acids with the synthesized PCR primers to obtain an amplified product;
- and
- (d) comparing the amplified product to a normal genotype encoding the normal HC gp-39L protein to detect any mutations in the amplified product.
2 more elements
A method of detecting altered expression of a HC gp-39L protein in a host comprising:
- (a) preparing a first antibody specific to a HC gp-39L protein;
- (b) preparing a reporter antibody against the first antibody, said reported antibody linked to an attached detectable reagent;
- (c) obtaining a sample from a host;
- (d) incubating said sample on a solid support which binds any proteins in said sample;
- (e) incubating said first antibody with any bound protein on said solid support so that said first antibody attaches to any HC ap-39L proteins bound to said solid support;
- (f) contacting said reporter antibody linked to a detectable reagent with said attached first antibody on said solid support;
- (g) detecting said detectable reagent;
- and
- (h) determining an amount of HC gp-39L protein in said sample.
6 more elements
Dependencies are inferred from the claim text.