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OwnerTeryl K. Frey, Konstantin V. Pugachev +2inventors
InventorsTeryl K. Frey, Konstantin V. Pugachev, Emily S. Abernathy, Wen-Pin Tzeng
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Highly infectious rubella virus cDNA clones derived from infectious cDNA clone having a low specific infectivity and methods of obtaining highly infectious rubella virus cDNA clones. Togavirus expression vectors of improved stability for the expression of live, attenuated togavirus and a foreign gene, based on the nucleic acid sequence of an infectious rubella virus clone and contain a togavirus non-structural protein open reading frame; an expression element for expression of a foreign gene; a foreign gene or a multiple cloning site for insertion of a foreign gene; an expression element for the expression of the live, attenuated togavirus; and a togavirus structural protein open reading frame. The expression element is either a subgenomic promoter or an internal ribosome entry site (IRES). Administration of the vector as an immunization agents is useful for the induction of immuity against the togavirus, the foreign gene, or both.
A chimeric nucleotide construct, comprising a DNA molecule encoding an infectious rubella virus having a specific infectivity of less than or equal to 0.5 plaques/μg of transcript, wherein portions of the DNA molecule have been replaced with one or more corresponding DNA fragments of a rubella virus genome, wherein the fragments have a minimal number of mutations, and wherein the chimeric nucleotide construct encodes a rubella virus having a specific infectivity of greater than 0.5 plaques/μg of transcript.
A method of producing a highly infectious rubella virus chimeric DNA molecule construct, comprising replacing a portion of a DNA molecule encoding an infectious rubella virus having a specific infectivity of less than 0.5 plaques/μg of transcript with one or more corresponding DNA fragments of a rubella virus genome, wherein the fragments have a minimal number of mutations, and wherein a specific infectivity of the resulting chimeric construct is greater than 0.5 plaques/μg of transcript.
A togavirus expression vector, comprising a togavirus non-structural protein open reading frame, a first expression element for expression of a foreign gene, operably linked to the foreign gene or a multiple cloning site for insertion of the foreign gene, and a second expression element, operably linked to a togavirus structural protein open reading frame, wherein the first expression element and the second expression element are, independently, a subgenomic promoter or an internatl ribosome entry site.
A method of producing a togavirus expression vector, comprising operably linking
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