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OwnerBob B. Buchanan, Boihon C. Yee +5inventors
InventorsBob B. Buchanan, Boihon C. Yee, Joshua H. Wong, Rosa M. Lozano, Karoly Kobrehel, Jin-An Jiao, Sungho Shin
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Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or E. coli. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.
A method of reducing a cystine containing, non-thionin protein comprising
A composition comprising a cystine containing, non-thionin protein, thioredoxin, NADP-thioredoxin reductase and NADPH or an NADPH generator composition.
A method of reducing an amylase inhibitor protein having disulfide bonds comprising
A composition comprising an amylase inhibitor protein having disulfide bonds, a thioredoxin, NADP-thioredoxin reductase and NADPH or an NADPH generator composition.
A method of reducing a protease inhibitor protein having disulfide bonds comprising
A method of reducing a gliadin containing a cystine group comprising
A method of reducing a glutenin comprising
A method for inactivating an enzyme inhibitor protein in a food product, said inhibitor containing cystine groups, comprising
A composition comprising thioredoxin, NADP-thioredoxin reductase, NADPH or an NADPH generator composition.
A method of improving the characteristics of a dough or a baked good comprising the steps of:
A method of improving the characteristics of a semolina dough or cooked pasta comprising the steps of:
A method of producing a dough from rice, corn, soybean, barley, oat, sorghum, cassava or millet flour, comprising
A method of producing a dough from rice, corn, soybean, barley, oat, sorghum or millet flour, comprising
A method for producing an improved gluten comprising
A method for producing an improved gluten comprising
A method for producing a gluten like product comprising
A dough mixture comprising a thiol redox protein, said thiol redox protein being from about 0.1 ppm to about 1.0 ppm of said mixture.
A yeast cell transformed with a vector containing a recombinant thioredoxin DNA.
A yeast cell transformed with a vector containing a recombinant NADP-thioredoxin reductase DNA.
A method for improving dough quality comprising
A method for improving dough quality comprising
A method for improving the quality of a baked good comprising
A method for improving the quality of a baked good comprising
A method of reducing the intramolelcular disulfide bonds of a non-thionin, non-chloroplast protein containing more than one intramolecular cystine comprising:
A composition comprising an intramolecular cystines containing non-thionin, non-chloroplast, plant protein, thioredoxin, NADP-thioredoxin reductase and NADPH or an NADPH generator composition.
A method of decreasing the heat or protease stability of a protein having intramolecular disulfide bonds comprising:
A method of selectively substantially reducing only the intramolecular disulfide bonds of a particular protein having intramolecular and intermolecular disulfide bonds comprising:
An isolated pullulanase inhibitor protein having disulfide bonds and a molecular weight of between 8 to 15 kDa.
A method of increasing the activity of pullulanase derived from barley or wheat endosperm comprising:
A method of improving the characteristics of a cooked pasta comprising the steps of:
A method of improving the characteristics of a dough or a baked good comprising the steps of:
A method of improving the characteristics of a baked good comprising the steps of:
A method of improving the characteristics of a triticale baked good comprising the steps of:
A method of reducing a snake neurotoxin protein having one or more intramolecular cystines comprising:
A composition comprising a snake neurotoxin protein and a thiol redox (SH) agent.
A method of reducing a snake neurotoxin protein having one or more intramolecular cystines comprising:
A method of altering the biological activity of a snake neurotoxin having one or more intramolecular cystines comprising
A method of inactivating, in vitro, a snake neurotoxin having one or more intramolecular cystines comprising adding a thiol redox (SH) agent to a liquid containing said toxin wherein said amount of said agent is effective for reducing said toxin.
A composition comprising a snake neurotoxin protein and a thiol redox (SH) agent in a liquid.
A method of inactivating, in vitro, a snake neurotoxin having one or more intramolecular cystines comprising adding to a liquid containing said toxin amounts of NADP-thioredoxin reductase, NADPH or an NADPH generator system and a thioredoxin effective for reducing said toxin thereby inactivating said toxin.
A composition comprising a liquid having an inactivated snake neurotoxin, NADP-thioredoxin reductase, NADPH or an NADPH generator system and a thioredoxin.
A method of treating snake venom neurotoxicity in an individual comprising administering, to an individual suffering from snake venom neurotoxicity amounts of a thiol redox (SH) agent effective for reducing or alleviating said snake venom neurotoxicity.
A method of treating snake venom neurotoxicity in an individual comprising administering, to an individual suffering from snake venom neurotoxicity, amounts of NADP-thioredoxin reductase, NADPH or an NADPH generator system and a thioredoxin effective for reducing or alleviating said snake venom neurotoxicity.
A method of reducing a bee venom toxic protein having one or more intramolecular cystines comprising:
A method of inactivating, in vitro, a bee venom having one or more intramolecular cystines comprising adding a thiol redox (SH) agent to a liquid containing said venom wherein said amount of said agent is effective for reducing said venom.
A method of treating bee venom toxicity in an individual comprising administering, to an individual suffering from bee venom toxicity amounts of a thiol redox (SH) agent effective for reducing or alleviating said bee venom toxicity.
A method of reducing a scorpion venom toxic protein having one or more intramolecular cystines comprising:
A method of inactivating, in vitro, a scorpion venom toxin having one or more intramolecular cystines comprising adding a thiol redox (SH) agent to a liquid containing said venom wherein said amount of said agent is effective for reducing said toxin.
A method of treating scorpion venom toxicity in an individual comprising administering, to an individual suffering from scorpion venom toxicity amounts of a thiol redox (SH) agent effective for reducing or alleviating said scorpion venom toxicity.
A method of treating bee venom toxicity in an individual comprising administering, to an individual suffering from bee venom toxicity, amounts of NADP-thioredoxin reductase, NADPH or an NADPH generator system and a thioredoxin effective for reducing or alleviating said bee venom toxicity.
A method of treating scorpion venom toxicity in an individual comprising administering, to an individual suffering from scorpion venom toxicity, amounts of NADP-thioredoxin reductase, NADPH or an NADPH generator system and a thioredoxin effective for reducing or alleviating said scorpion venom toxicity.
Dependencies are inferred from the claim text.