Fabrication of protein gel for new application
OwnerJiunn Yeong Teoinventor
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Abstract
This invention relates to a method of forming protein gel matrices for separation, immobilization, diagnosis, and artificial organs. These matrices are made of proteins and are different from their polymeric counterparts. The formation of supported or non-supported flat sheet membranes, supported or non-supported tubular membranes, hollow fiber membranes, monoliths for continuous bed chromatography, enzyme-linked immuno sorbents, gas sorbents, liquid sorbents, edible sorbents, hemoperfusion sorbents, and surgical sponges are also disclosed.
Claims16
A method of making protein matrix for separation, immobilization, diagnosis, and artificial organ, comprising the steps of:
- a) Preparing a homogeneous protein solution by dissolving the following components in water:
- i. Proteins or mixtures of different proteins selected from whey protein isolate, whey protein concentrate, ovalbumin, casine, beta-lactoglobulin, alpha-lactalbumin, or mixture thereof, at a total protein concentration of 5 to 80 weight percents in the final protein solution;
- ii. Metal salts or mixture of different metal salts at a total salt concentration of 0.001M to 0.5M in the final protein solution;
- iii. Acid, base, or mixtures thereof to change the pH value of the protein solution to between 2 and 11.
- b) Degasing the protein mixtures in Step 1a by centrifugation, vacuuming, sonication, or combinations thereof, to remove the trapped air in the protein solution.
- c) Extruding, spinning or casting the protein mixtures in Step 1b into a protein matrix of any size and shape at predetermined conditions;
- d) Strengthening the protein matrix through a heat treatment or a series of heat treatments, including steam heating and baking, at predetermined temperature or temperatures, pressure or pressures, and humidity or humidities for predetermined length of time to effectively strengthen the protein matrix.
Dependencies are inferred from the claim text.