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AssigneeIschemia Technologies, Inc.
InventorsDavid Bar-Or, Edward Lau, James V. Winkler
Estimating the term…
The present invention relates to rapid methods for the detection of ischemic states and to kits for use in such methods. Provided for is a rapid method of testing for and quantifying ischemia based upon methods of detecting and quantifying the existence of an alteration of the serum protein albumin which occurs following an ischemic event; methods for detecting and quantifying this alteration include evaluating and quantifying the cobalt binding capacity of circulating albumin, analysis and measurement of the ability of serum albumin to bind exogenous cobalt, detection and measurement of the presence of endogenous copper in a purified albumin sample and use of an immunological assay specific to the altered form of serum albumin which occurs following an ischemic event. Also taught by the present invention is the detection and measurement of an ischemic event by measuring albumin N-terminal derivatives that arise following an ischemic event, including truncated albumin species lacking one to four N-terminal amino acids or albumin with an acetylated N-terminal Asp residue.
A diagnostic kit for an ischemic event comprising a solid support comprising:
The kit of claim 13, wherein said metal ion is cobalt ion.
The kit of claim 13, further comprising an end of process indicator at the second end of said solid support.
The kit of claim 13, further comprising a second elongated solid support having a first and second end, said second support first end and said first support first end sharing said application site, and having an area of immobilized ligand to non-N-terminal albumin epitope and/or derivative proximate the second end, said ligand of said area proximate the second end being the same as the ligand in the second area of the first elongated support, and said second support serving as a control.
Dependencies are inferred from the claim text.