Enhanced meristematic activity and competence by overexpression of tonoplast pyrophosphatase

A method of making a transgenic plant with increased meristematic competence comprising introducing into one or more cells of a plant exogenous nucleic acid which alters expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the plant to yield transformed cells in the plant, thereby producing a transgenic plant which has increased meristematic competence.

A method of making a transgenic plant with increased biomass comprising introducing into one or more cells of a plant a nucleic acid construct which alters. expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the cell to yield transformed cells thereby producing a transgenic plant with increased biomass.

A method of making a transgenic plant with an increased biomass over its corresponding wild type plant, wherein the increased biomass relates to an increase in the biomass of a plant part selected from the group consisting of:

• leaves, stems, roots, seeds, flowers, and fruits, said method comprising introducing into one or more cells of a plant, an exogenous nucleic acid which alters expression of vacuolar pyrophosphatase such so as to enhance the activity of the vacuolar pyrophosphatase in the plant to yield transformed cells, thereby producing a transgenic plant with an increased biomass.

A method of producing a transgenic plant with a stem structure thicker than wild type comprising introducing into one or more cells of a plant exogenous nucleic acid which alters expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the plant to yield transformed cells, thereby producing a transgenic plant with a thickened stem structure.

A method of making a transgenic plant having increased root structure compared to its corresponding wild type plant comprising introducing into one or more cells of a exogenous nucleic acid which alters expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the plant to yield transformed cells, thereby producing a transgenic plant having increased root structure.

A method of making a transgenic plant having increased shoot regeneration capacity as compared to its corresponding wild type plant comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the plant to yield transformed cells, thereby producing a transgenic plant having increased shoot regeneration capacity.

A method of making a transgenic plant having increased root regeneration capacity as compared to its corresponding wild type plant comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase so as to increase vacuolar pyrophosphatase activity in the plant to yield transformed cells, thereby producing a transgenic plant having increased root structure.