Human serine racemase
OwnerThomas M Connolly, Yuan Liu +1inventors
InventorsThomas M Connolly, Yuan Liu, Menghang Xia
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Abstract
The present invention provides polynucleotides and polypeptides of a human serine racemase. The polynucleotides and polypeptides are used to further provide expression vectors, host cells comprising the vectors, probes and primers, antibodies against the serine racemase protein and polypeptides thereof, assays for the presence or expression of serine racemase and assays for the identification of compounds that interact with serine racemase and transgenic animals expressing human serine racemase.
Claims14
A recombinant polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:2.
- (b) a polynucleotide having the nucleotide sequence of SEQ ID NO:1,
- (c) a polynucleotide which is complementary to the polynucleotide of (a) or (b), and
- (d) a polynucleotide that hybridizes with a polynucleotide of (a), (b), or (c) under stringent conditions.
An expression vector that directs the expression of a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:2.
- (b) a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 1,
- (c) a polynucleotide which is complementary to the polynucleotide of (a) or (b), and
- (d) a polynucleotide that hybridizes with a polynucleotide of (a), (b), or (c) under stringent conditions.
A recombinant polypeptide having an amino acid sequence of SEQ ID NO:2.
A method of determining whether a candidate compound is an inhibitor of a serine racemase polypeptide comprising:
- (a) providing at least one host cell harboring an expression vector that includes a polynucleotide selected from the group consisting of:
- (i) a polynucleotide encoding a polypeptide having an amino acid sequence of SEQ ID NO:2, and
- (ii) a polynucleotide having the coding sequence from SEQ ID NO:
- 1,
- (b) contacting at least one of said cells with the candidate to permit the interaction of the candidate with the serine racemase polypeptide, and
- (c) determining whether the candidate is an inhibitor of the serine racemase polypeptide by ascertaining the relative activity of the polypeptide in the presence of the candidate.
A transgenic animal lacking a functional endogenous serine racemase gene.
The animal of claim 12 further comprising a human serine racemase gene.
Dependencies are inferred from the claim text.