Loading patent…
AssigneeUniversity of Pittsburgh
InventorsLeaf Huang, Xiang Gao, Frank L. Sorgi, Ralph Wilfred Paul, David L. Sloane, Aaron Garth Loomis
Estimating the term…
Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method and upon purification, the complexes produced by the method of this invention are 50 to 500 fold more concentrated than the complexes formed by admixture. The method described herein provides for the large scale production of lipid-comprising drug delivery systems useful for gene therapy and other applications.
A drug/lipid complex comprising:
A method for producing drug/lipid complexes having a positive charge excess of lipid to drug, said method comprising:
A method for producing drug/lipid/polycation complexes having a positive charge excess of lipid and polycation to drug, said method comprising:
A drug/lipid/polycationic polypeptide complex comprising drug, at least one lipid species, and at least one polycationic polypeptide in a ratio such that said complex has a positive charge excess of lipid and polycationic polypeptide to drug.
A method for producing drug/lipid/polycationic polypeptide complexes, said method comprising mixing drug to lipid to polycationic polypeptide in a ratio of from about 1 μg/0.1 nmol/0.01 μg to about 1 μg/200 nmol/100 μg.
A polycationic polypeptide nucleic acid complex, comprising nucleic acid and at least one polycationic polypeptide in a ratio of about 1 μg nucleic acid to about 0.1 μg to about 10 μg of polycationic polypeptide.
Dependencies are inferred from the claim text.