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US 2003/0211559 A1Published application
Preparations of gamma-secretase activity
AssigneeScios, Inc.
InventorsBarbara L. Cordell, Jeffrey N. Higaki, Mitchell Mutz
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AssigneeScios, Inc.
InventorsBarbara L. Cordell, Jeffrey N. Higaki, Mitchell Mutz
Estimating the term…
The present invention provides cell-free γ-secretase activity. The method of the invention utilizes a membrane source of APP/γ-secretase mixture in the assay to determine factors that may enhance or decrease enzymatic activity affecting β-amyloid peptide production. The cell membranes used in the assay may be from cells expressing an endogenous APP or, preferably, cells expressing a recombinant APP. The APP may be full-length or a fragment capable of being proteolytically cleaved by γ-secretase. In addition, the APP expressed in the cells may have one or more mutation, such as a point mutation, small deletion, etc.
An isolated preparation of γ-secretase activity comprising a APP/γ-secretase mixture, wherein said activity is characterized by the ability to produce Aβ in vitro.
A method of identifying a compound characterized by an ability to alter γ-secretase activity, said method comprising the steps of:
A method of identifying a compound characterized by the ability to alter γ-secretase activity, said method comprising the steps of:
Dependencies are inferred from the claim text.