Human Eag2
AssigneeIcagen, Inc.
InventorsTimothy J. Jegla, Yi Liu
Life of this patent
Estimating the term…
Abstract
The invention provides isolated nucleic acid and amino acid sequences of Eag2, antibodies to Eag2, methods of detecting Eag2, and methods of screening for modulators of Eag2 potassium channels using biologically active Eag2. The invention further provides, in a computer system, a method of screening for mutations of human Eag2 genes as well as a method for identifying a three-dimensional structure of Eag2 polypeptide monomers.
Claims45
An isolated nucleic acid encoding an alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having the characteristic of voltage sensitivity;
- and
- (ii) comprises an amino acid sequence that has greater than 70% identity to amino acids 720-988 of a human Eag2 amino acid sequence.
An isolated nucleic acid encoding an alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having the characteristic of voltage sensitivity;
- and
- (ii) comprises an amino acid sequence that has greater than 85% identity to the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid encoding an Eag2 polypeptide, the nucleic acid specifically hybridizing under stringent conditions to a nucleotide sequence of SEQ ID NO:1.
An isolated nucleic acid encoding an Eag2 polypeptide, wherein the isolated nucleic acid specifically hybridizes to a nucleic acid encoding an amino acid of SEQ ID NO:2.
An isolated alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having a characteristic of voltage sensitivity;
- and
- (ii) comprises an amino acid sequence that has greater than 70% amino acid identity to amino acids 720-988 of a human Eag2 amino acid sequence.
An isolated alpha subunit of a potassium channel, wherein the subunit:
- (i) forms, with at least one additional Eag family alpha subunit, a potassium channel having a characteristic of voltage sensitivity;
- and
- (ii) comprises an amino acid sequence that has greater than 85% amino acid identity to the amino acid sequence of SEQ ID NO:2.
A method for identifying a compound that increases or decreases ion flux through an Eag potassium channel, the method comprising the steps of:
- (i) contacting the compound an alpha subunit of a potassium channel, wherein the subunit:
- (a) forms, with at least one additional Eag family alpha subunit, a potassium channel having a characteristic of voltage sensitivity;
- and
- (b) comprises an amino acid sequence that has greater than 70% identity to amino acids 720-988 of a human Eag2 amino acid sequence;
- and
- (ii) determining the functional effect of the compound upon the potassium channel.
A method for identifying a compound that increases or decreases ion flux through an Eag potassium channel, the method comprising the steps of:
- (i) contacting the compound an alpha subunit of a potassium channel, wherein the subunit:
- (a) forms, with at least one additional Eag family alpha subunit, a potassium channel having a characteristic of voltage sensitivity;
- and
- (b) comprises an amino acid sequence that has greater than 85% identity to an amino acid sequence of SEQ ID NO:2;
- and
- (ii) determining the functional effect of the compound upon the potassium channel.
A method for identifying a compound that increases or decreases ion flux through a potassium channel comprising an Eag2 polypeptide, the method comprising the steps of:
- (i) entering into a computer system an amino acid sequence of at least 50 amino acids of an Eag2 polypeptide or at least 150 nucleotides of a nucleic acid encoding the Eag2 polypeptide, the Eag2 polypeptide comprising a subsequence having at least 70% amino acid sequence identity to amino acids 720 to 988 of SEQ ID NO:2 or at least 85% amino acid sequence identity to an amino acid sequence of SEQ ID NO:2;
- (ii) generating a three-dimensional structure of the polypeptide encoded by the amino acid sequence;
- (iii) generating a three-dimensional structure of the potassium channel comprising the Eag2 polypeptide;
- (iv) generating a three-dimensional structure of the compound;
- and
- (v) comparing the three-dimensional structures of the polypeptide and the compound to determine whether or not the compound binds to the polypeptide.
A method of detecting the presence of human Eag2 in a biological sample, the method comprising the steps of:
- (i) isolating a biological sample;
- (ii) contacting the biological sample with a human Eag2-specific reagent that selectively associates with human Eag2;
- and,
- (iii) detecting the level of human Eag2-specific reagent that selectively associates with the sample.
In a computer system, a method of screening for mutations of a human Eag2 gene, the method comprising the steps of:
- (i) entering into the system at least about 50 nucleotides of a first nucleic acid sequence encoding a human Eag2 gene having a nucleotide sequence of SEQ ID NO:
- 1, and conservatively modified variants thereof;
- (ii) comparing the first nucleic acid sequence with a second nucleic acid sequence having substantial identity to the first nucleic acid sequence;
- and
- (iii) identifying nucleotide differences between the first and second nucleic acid sequences.
Dependencies are inferred from the claim text.