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US 2003/0211509 A1Published application
TNF-delta ligand and uses thereof
OwnerSteven R. Wileyinventor
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OwnerSteven R. Wileyinventor
Estimating the term…
An isolated clone consisting of sequences transcribed from the TNF-delta gene is disclosed. Also provided are human polypeptides translated from said TNF-delta sequences and a procedure for producing such polypeptide by recombinant techniques. Also provided are a procedure for producing soluble biologically active TNF-delta, which may be used to treat deficiencies of TNF-delta and diseases conditions ameliorated by TNF-delta. Antibodies, antagonists and inhibitors of such polypeptide which may be used to prevent the action of such polypeptide and therefore may be used therapeutically to treat TNF-delta associated diseases, tumors or metastastases are disclosed. Also disclosed is the use of said antibodies, agonists and inhibitors as well as the nucleic acid sequences to screen for, diagnose, prognosticate, stage and monitor conditions and diseases attributable to TNF-delta, especially inflammation. The use of said partial sequence to provide antibodies, agonists and inhibitors as well as partial nucleic acid sequences to screen for, diagnose, stage and monitor diseases associated with TNF-delta, including but not limited to inflammation is also disclosed. Illustrative sequences and clone designations for TNF-delta are provided.
A method of detecting the presence of target polynucleotide of TNF-delta in a test sample, comprising:
A method for detecting mRNA of TNF-delta in a test sample, comprising:
A method of detecting target TNF-delta polynucleotide in a test sample suspected of containing said target, comprising:
A test kit useful for detecting TNF-delta polynucleotide in a test sample, comprising a container containing at least one TNF-delta polynucleotide having at least 50% identity to SEQUENCE ID NO 1 and fragments, analogs or complements thereof.
A purified polynucleotide or fragment thereof derived from TNF-delta gene wherein said purified polynucleotide is capable of selectively hybridizing to the nucleic acid of said TNF-delta gene, and wherein said purified polynucleotide has at least 50% identity to SEQUENCE ID NO 1 and fragments, analogs or complements thereof.
A recombinant expression system comprising a nucleic acid sequence that encodes an open reading frame derived from TNF-delta which is operably linked to a control sequence compatible with a desired host and wherein said nucleic acid sequence has at least 50% identity to SEQUENCE ID NO 1 and fragments, analogs or complements thereof.
A polypeptide encoded by TNF-delta, wherein said polypeptide has at least 35% identity to amino acid sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
A method for determining whether a compound is an agonist or antagonist to TNF-delta protein, comprising:
A method for determining whether a receptor binds to a TNF-delta ligand, comprising:
An antibody which specifically binds to at least one epitope encoded by TNF-delta, wherein said antibody is polyclonal or monoclonal and wherein said epitope comprises an amino acid sequence having at least 35% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
An assay kit for determining the presence of TNF-delta antigen or antibody in a test sample, comprising a container containing a TNF-delta polypeptide having at least 35% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
An assay kit for determining the presence of TNF-delta antigen or antibody in a test sample, comprising a container containing an antibody which specifically binds to TNF-delta antigen, wherein said antigen comprises at least one epitope of TNF-delta having at least about 60% similarity to a sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
A method for producing a polypeptide comprising at least one epitope of TNF-delta, which method comprises incubating host cells transformed with an expression vector, wherein said vector comprises a polynucleotide sequence encoding a polypeptide, which polypeptide comprises an amino acid sequence having at least 35% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
A method for detecting TNF-delta antigen in a test sample suspected of containing said TNF-delta antigen, comprising:
A method for detecting antibodies which bind to TNF-delta antigen in a test sample suspected of containing said antibodies, comprising:
A tissue culture grown cell comprising a nucleic acid sequence that encodes at least one epitope of TNF-delta antigen or a fragment thereof, wherein said nucleic acid sequence is transfected into said cell and wherein said nucleic acid sequence is SEQUENCE ID NO 1 and fragments, analogs or complements thereof.
A method for producing antibodies which specifically bind to TNF-delta antigen, comprising administering to an individual an isolated immunogenic polypeptide or fragment thereof, wherein said isolated immunogenic polypeptide comprises at least one TNF-delta epitope and has at least 35% identity to a sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof, in an amount sufficient to produce an immune response.
A method for producing antibodies which specifically bind to TNF-delta antigen, comprising administering to a mammal a plasmid comprising a TNF-Delata sequence which encodes at least one epitope of TNF-delta, where said TNF-delta sequence is selected from the group consisting of SEQUENCE ID NO 1 and fragments or complements thereof.
A composition of matter comprising a TNF-delta polynucleotide or fragment thereof, wherein said polynucleotide has at least 50% identity to SEQUENCE ID NO 1 and fragments, analogs or complements thereof.
A composition of matter comprising a polypeptide containing at least one epitope encoded by TNF-delta wherein said polypeptide has at least 35% identity to a sequence selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3 and fragments thereof.
A gene or fragment thereof which codes for TNF-delta protein, wherein said TNF-delta protein comprises an amino acid sequence which has at least 35% identity to SEQUENCE ID NO 2 or SEQUENCE ID NO 3.
A gene or fragment thereof comprising DNA having at least 35% identity to SEQUENCE ID NO 1.
Dependencies are inferred from the claim text.