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OwnerHuimin Kong, Lauren Sears Higgins +4inventors
InventorsHuimin Kong, Lauren Sears Higgins, Michael A Dalton, Rebecca B Kucera, Ira Schildkraut, Geoffrey G Wilson
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The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI and other nicking enzymes in non-modified strand displacement amplification, is disclosed also.
Isolated DNA coding for the N.BstNBI restriction endonuclease, wherein the isolated DNA is obtainable from ATCC Accession No. PTA-1925.
Isolated DNA coding for the PleI methylase, wherein the isolated DNA is obtainable from ATCC Accession No. Pta-1925.
A vector comprising isolated DNA selected from the group consisting essentially of SEQ ID NO:
A method for strand displacement amplification in the absence of modified nucleotide comprising employing a restriction endonuclease which does not require modified nucleotides to nick double-stranded DNA on a single DNA strand.
Isolated DNA coding for the N.BstNBI DNA methylase, wherein the isolated DNA is obtainable from ATCC Accession No. PTA-1925.
A method of making a mutated Type IIT endonuclease which has nicking activity comprising the steps of:
Dependencies are inferred from the claim text.