Transcription factor profiling on a solid surface
AssigneeGenTel Corporation
InventorBryce P. Nelson
Life of this patent
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Abstract
The present invention relates to novel methods for the analysis of interactions of transcription factors with target nucleic acids. In particular, the present invention relates to compositions and methods for the detection of transcription factors binding to their target promoter regions. The present invention further provides methods of screening compounds for their ability to alter such binding interactions.
Claims90
A composition comprising an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets.
A composition comprising an arrayed solid surface comprising a plurality of microfluidics channels, said solid surface comprising an array of transcription factor binding targets.
A composition comprising an arrayed solid surface comprising a plurality of etched microchannels, said solid surface comprising an array of transcription factor binding targets.
A composition comprising an arrayed solid surface comprising an array of transcription factor binding targets in contact with a biological sample containing at least one transcription factor.
A composition comprising an arrayed solid surface comprising an array of transcription factor binding targets in contact with a biological sample containing at least one transcription factor, wherein said biological sample has been treated with a small molecule.
A composition comprising an arrayed solid surface comprising an array of transcription factors.
A composition comprising an arrayed solid surface comprising an array of transcription factors in contact with a sample comprising at least one transcription factor binding target.
A composition comprising an arrayed solid surface comprising an array of transcription factors in contact with a treated biological sample, said treated biological sample used to prepare a cell lysate containing at least one test compound.
A system, comprising a composition comprising an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets;
- at least one transcription factor polypeptide;
- and a detection apparatus in communication with said arrayed solid surface.
A system, comprising a composition comprising an arrayed solid surface, said solid surface comprising an array of transcription factor polypeptides;
- at least one transcription factor binding target;
- and a detection apparatus in communication with said arrayed solid surface.
A system, comprising an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets;
- and a biological sample containing at least one transcription factor, said biological sample in communication with said array solid surface.
A system, comprising an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets;
- and a biological sample containing at least one transcription factor, said biological sample in communication with said array solid surface, and wherein said biological sample has been treated with a small molecule.
A system, comprising a composition comprising an arrayed solid surface, said solid surface comprising an array of transcription factor polypeptides;
- at least one transcription factor binding target;
- and a cell lysate comprising at least one test compound, and said cell lysate in contact with said array solid surface.
A method of detecting biomolecular interactions, comprising:
- a) providing
- i) an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets;
- ii) at least one transcription factor polypeptide;
- and
- iii) a detection apparatus in communication with said arrayed solid surface;
- and
- b) contacting said at least one transcription factor polypeptide with said array of transcription factor targets under conditions such that said apparatus detects interactions between said array of transcription factor targets and said at least one transcription factor polypeptide.
A method of detecting biomolecular interactions, comprising:
- a) providing
- i) an arrayed solid surface, said solid surface comprising an array of transcription factors;
- ii) at least one transcription factor binding target;
- and
- iii) a detection apparatus in communication with said arrayed solid surface;
- and
- b) contacting said at least one transcription factor binding target with said array of transcription factors under conditions such that said apparatus detects interactions between said array of transcription factors and said at least one transcription factor binding target.
A method for measuring biomolecular interactions, comprising:
- a) providing
- i) an arrayed solid surface, said solid surface comprising an array of transcription factor binding targets;
- ii) a biological sample containing at least one transcription factor;
- and
- iii) a detection apparatus;
- and
- b) contacting said biological sample with said arrayed solid surface targets under conditions such that said apparatus detects interactions between said array of transcription factor targets and said at least one transcription factor contained in said biological sample.
A method for measuring the effect of small molecules on biomolecular interactions, the method comprising:
- a) providing
- i) an arrayed surface, said surface comprising an array of transcription factor binding targets;
- ii) a test compound;
- iii) a biological sample comprising at least one transcription factor;
- iv) a detection apparatus;
- and
- b) treating said biological sample with said small molecule;
- contacting said treated biological sample with said array of transcription factor targets under conditions such that said apparatus detects interactions between said array of transcription factor targets and said at least one transcription factor contained in said biological sample.
A method for measuring biomolecular interactions, comprising:
- a) providing
- i) an arrayed solid surface, said solid surface comprising an array of transcription factors;
- ii) a biological sample comprising at least one test compound;
- iii) at least one transcription factor binding target;
- and
- iv) a detection apparatus;
- and
- b) contacting said biological sample and said at least one binding target with said arrayed solid surface under conditions such that said detection apparatus detects interactions between said array of transcription factors and said at least one transcription factor binding target.
A method for measuring the effect of small molecules on biomolecular interactions, comprising:
- a) providing
- i) an arrayed solid surface, said surface comprising an array of transcription factor binding targets;
- ii) at least one small molecule test compound;
- iii) a biological sample containing at least one transcription factor;
- iv) a detection apparatus;
- and
- b) treating said biological sample with said at least one small molecule test compound to generate a treated biological sample;
- c) contacting said treated biological sample with said array of transcription factor targets under conditions such that said apparatus detects interactions between said array of transcription factor targets and said at least one transcription factor contained in said biological sample.
Dependencies are inferred from the claim text.