Fertility associated antigens and method of use thereof
OwnerHuanmin Zhang, Roy L Axinventors
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Abstract
The present invention relates to a gene encoding a fertility associated antigen methods of employing the fertility associated antigen to stabilize sperm and/or increase the fertility of a male and method of assessing, the fertility and/or reproductive fitness of a male
Claims69
An isolated polynucleotide comprising the nucleic acid sequence shown in SEQ ID NO:
- 2, 3, 4, or 5.
An isolated polypeptide comprising the amino acid sequence in SEQ ID NO:
- 7, 8, 9, or 10.
A method of increasing the stability of a sperm cell acrosome comprising
- administering a fertility-associated antigen comprising the amino acid sequence in SEQ ID NO:
- 7, 8, 9 or 10 to the sperm cell in an amount sufficient to increase the stability of said sperm cell.
A method of increasing the fertility of a human male comprising
- (a) isolating sperm from said human male;
- and
- (b) administering a fertility associated antigen comprising the amino acid sequence in SEQ ID NO:
- 7, 8, 9 or 10 in an amount sufficient to increase the fertility of said human male.
An isolated polynucleotide comprising the nucleic acid sequence shown in SEQ ID NO:
- 1.
An isolated polypeptide comprising the amino acid sequence in SEQ ID NO:
- 6.
A method of increasing the stability of a sperm cell acrosome comprising
- administering a fertility-associated antigen comprising the amino acid sequence in SEQ ID NO:
- 6 to the sperm cell in an amount sufficient to increase the stability of said sperm cell.
The method of claim 44, wherein said administering is in vitro.
A method of increasing the fertility of a male mammal comprising
- (c) isolating sperm from said male;
- and
- (d) administering a fertility associated antigen comprising the amino acid sequence in SEQ ID NO:
- 6 in an amount sufficient to increase the fertility of said human male.
A method of assaying the fertility of a mammal comprising
- (a) detecting the presence or absence of a FAA SNP in said mammal;
- and
- (b) correlating said presence or absence with the fertility of said mammal.
A oligonucleotide probe for assessing the fertility of a mammal which comprises at least 15 nucleotides that binds under suitably stringent conditions to a FAA SNP polynucleotide and which does not hybridize to a FAA which does not contain said SNP.
An assay kit comprising a first oligonucleotide probe for assessing the fertility of a mammal which comprises at least 15 nucleotides that binds under suitably stringent conditions to a FAA SNP polynucleotide and which does not hybridize to a FAA which does not contain said SNP;
- and at least one additional oligonucleotide probe which is different from the first oligonucleotide probe and which comprises at least 15 nucleotides that binds under suitably stringent conditions to a FAA SNP polynucleotide and which does not hybridize to a FAA which does not contain said SNP including said first FAA SNP.
A method of detecting a FAA SNP comprising
- (a) obtaining a sample which has a polynucleotide which comprises a FAA SNP;
- (b) amplifying said polynucleotide with at least two oligonucleotide primers which comprise at least 15 nucleotides of the nucleotide sequence in SEQ ID NO:
- 2, 3, 4 or 5, wherein said oligonucleotide primers oligonucleotide primers amplify the FAA SNP;
- and
- (c) detecting the presence or absence of said FAA SNP.
A method of detecting a FAA SNP comprising
- (d) obtaining a sample which has a polynucleotide which comprises a FAA SNP;
- (e) amplifying said polynucleotide with at least two oligonucleotide primers which comprise at least 15 nucleotides of the nucleotide sequence in SEQ ID NO:
- 1,
- wherein said oligonucleotide primers amplify the FAA SNP;
- and detecting the presence or absence of said FAA SNP.
A method of assaying the fertility of a mammal comprising
- (a) detecting the presence or absence of a FAA SNP in said mammal;
- and
- (b) correlating said presence or absence with the fertility of said mammal.
A method of determining the association of a FAA SNP with the reproductive fitness of a mammal comprising:
- (a) determining the nucleotide sequence of said FAA SNP;
- (b) quantifying the fertility of more than one mammal containing said FAA SNP and
- (c) correlating the frequency of said FAA SNP to the reproductive fitness of said mammals.
Dependencies are inferred from the claim text.