Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo
OwnerGregory Henry Reverainventor
Life of this patent
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Abstract
Methods to create human stem cells without creating or destroying a human embryo have been developed. The invention includes a method of creating a human stem cell by obtaining a non-human stem cell and removing the nucleus. The nucleus of a human donor cell is transferred to the non-human stem cell.
Claims30
A method of creating a human stem cell, comprising:
- (a) transferring the nucleus of a human somatic donor cell into an enucleated non-human mammalian stem cell forming a nuclear transfer unit;
- and
- (b) activating the nuclear transfer unit.
A method of creating a human stem cell, comprising:
- (a) enucleating a non-human mammalian stem cell;
- and
- (b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.
A method of creating a human stem cell, comprising:
- (a) enucleating a non-human mammalian stem cell;
- (b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell forming a nuclear transfer unit;
- and
- (c) activating the nuclear transfer unit.
A method of creating a human stem cell, comprising:
- (a) enucleating a human oocyte;
- (b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;
- (c) activating the first nuclear transfer unit.
A method of creating a human stem cell;
- comprising:
- (a) enucleating a human oocyte;
- (b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;
- (c) activating the first nuclear transfer unit;
- (d) culturing the first nuclear transfer unit to yield at least one non-human stem cell;
- (e) enucleating the non-human stem cell;
- and
- (f) transferring the nucleus of a human somatic donor cell into the enucleated non-human stem cell to create a second nuclear transfer unit;
A method of creating a human stem cell, comprising:
- (a) enucleating a human oocyte;
- (b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte creating a first nuclear transfer unit;
- (c) activating the first nuclear transfer unit;
- (d) culturing the first nuclear transfer unit to yield at least one non-human stem cell;
- (e) enucleating the non-human stem cell;
- (f) transferring the nucleus of a human somatic donor cell into the enucleated non-human stem cell to create a second nuclear transfer unit;
- and
- (g) activating the second nuclear transfer unit.
A method of creating a human stem cell, comprising:
- (a) step for transferring the nucleus of a human somatic donor cell into an enucleated non-human mammalian stem cell forming a nuclear transfer unit;
- and
- (b) step for activating the nuclear transfer unit.
A method of creating a human stem cell, comprising:
- (a) step for enucleating a non-human mammalian stem cell;
- and
- (b) step for transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.
A method of creating a human stem cell, comprising:
- (a) step for enucleating a human oocyte;
- (b) step for transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated human oocyte.
Method for creating a nuclear transfer unit comprising:
- (a) enucleating a non-human mammalian stem cell;
- and
- (b) transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.
Method for creating a nuclear transfer unit comprising:
- (a) step for enucleating a non-human mammalian stem cell;
- and
- (b) step for transferring the nucleus of a human somatic donor cell into the enucleated non-human mammalian stem cell.
Method for creating a nuclear transfer unit comprising:
- (a) enucleating a human oocyte;
- and
- (b) transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated oocyte.
Method for creating a nuclear transfer unit comprising:
- (a) step for enucleating a human oocyte;
- and
- (b) step for transferring the nucleus of a non-human mammalian somatic donor cell into the enucleated oocyte.
Dependencies are inferred from the claim text.