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US 2003/0207343 A1Published application
Method for determining the anticoagulatory potential of a sample
AssigneeDade Behring Marburg GmbH
InventorMichael Kraus
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Abstract
The application relates to a method for determining the anticoagulatory potential of a sample by adding thrombomodulin and thromboplastin in a coagulation test.
Claims36
1) A method for determining the anticoagulatory potential of a sample in the presence of exogenously added thrombomodulin, which method includes the following steps:
- a) the following reagents are added to the sample, preferably a plasma sample:
- i) exogenous thrombomodulin which can form a complex with thrombin, with this complex being able to activate the protein C in the sample, and with it being possible for the protein C to be endogenous protein C or exogenously added protein C,
- ii) an activator which leads, without any further intermediate incubation, to the activation of thrombin, with it being possible for the prothrombin to be endogenous prothrombin or exogenously added prothrombin,
- iii) phospholipids,
- iv) calcium ions,
- v) and also other additional reagents which are used generally for optimizing coagulation tests,
- b) the reaction is started by adding the prothrombin activator-containing reagent, and
- c) the formation of thrombin is determined by measuring the transformation rate of a thrombin substrate, with this transformation rate being determined by measuring the time until a fibrin clot has formed or by the transformation rate of a labeled thrombin substrate.
5 more elements
35) A method for determining the AT III activity and the protein C system activity of a sample in the presence of exogenously added thrombomodulin, which method includes the following steps:
- a) the following reagents are added to the sample, preferably a plasma sample:
- i) exogenous thrombomodulin which, in addition to its protein C-activating activity (PCaA), also possesses the property of accelerating the inhibition of thrombin by antithrombin III (AITA), or to which heparin is added in order to reconstitute the AITA property,
- ii) at least one activator which leads, without any further intermediate incubation, to the activation of prothrombin to form thrombin, with it being possible for the prothrombin to be endogenous prothrombin or exogenously added prothrombin,
- iii) phospholipids,
- iv) calcium ions,
- v) and also other additional reagents which are used generally for optimizing coagulation tests,
- b) the formation of thrombin is determined by measuring the transformation rate of a thrombin substrate, with this transformation rate being determined by measuring the time until a fibrin clot has formed or by the transformation rate of a labeled thrombin substrate.
4 more elements
Dependencies are inferred from the claim text.