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US 2003/0207302 A1Published application
Universal probe system
OwnerColin Potterinventor
Life of this patent
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OwnerColin Potterinventor
Estimating the term…
A method for detecting a target nucleic acid in a sample, which method comprises: (a) providing a primer air comprising a first primer having a target specific sequence complementary to the sequence of the first strand of said target nucleic acid and an overhang sequence unrelated to said target nucleic acid sequence and a second primer having a target specific sequence complementary to the sequence of the second strand of said target nucleic acid and an attachment means; (b) contacting a nucleic acid sample with said first and second primers; (c) carrying out a polymerase chain reaction (PCR) under conditions suitable for the formation of a product having said attachment means and a sequence comprising the sequence of said target nucleic acid and the sequence of said overhang; (j) separating the first strand of said product comprising said first primer from the second strand of said product comprising said second primer using said attachment means; and (k) detecting said first strand or said second strand thereby detecting whether said target nucleic acid is present in said sample.
A method for detecting a target nucleic acid in a sample, which method comprises:
A method according to any one of the preceding claims, wherein detection in step (e) is carried out using a probe comprising a sequence identical to the sequence of the overhang in said first primer.
A method according to any one of the preceding claims, wherein said second primer comprises an overhang sequence.
A method according to any one of the preceding claims, wherein said attachment means on said second primer comprises a biotin moiety.
A method according to any one of the preceding claims, wherein said first primer further comprises an attachment means.
A method according to any one of the preceding claims, wherein a set of primers comprising two or more said primer pairs are provided for the detection of two or more target nucleic acids.
A method according to any one of the preceding claims for use in determining the presence of a single nucleotide polymorphism (SNP) in a sample from an individual.
A method according to any one of the preceding claims, wherein:
A primer pair suitable for use in detecting a target nucleic acid, which primer pair comprises:
A kit for detecting a target nucleic acid molecule, which kit comprises:
A PCR product having a sequence comprising:
Dependencies are inferred from the claim text.