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US 2003/0207256 A1Published application
Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices
AssigneeKimberly-Clark Worldwide, Inc.
InventorsCurtis Sayre, David Cohen, Rosann Kaylor
Life of this patent
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Abstract
A biosensor includes a substrate with a layer of receptive material disposed thereon overlying a layer containing a photo-reactive agent. The receptive material is specific for an analyte of interest. A pattern of active and inactive areas of the receptive material are defined in the receptive material layer by a masking process wherein the photo-reactive agent is activated in the exposed regions of the mask.
Claims25
A biosensor, comprising:
- a substrate member;
- a photo-reactive agent at a surface of said substrate member;
- a receptive material layer applied over said photo-reactive agent and generally uniformly covering said side of said substrate member, said receptive material being specific for an analyte of interest;
- a pattern of active and inactive areas of said receptive material defined on said layer, said active and inactive areas formed by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive agent such that areas exposed by said mask define said pattern of inactive areas of said receptive material and said areas shielded by said mask define said pattern of active areas of receptive material;
- and
- wherein when said biosensor is exposed to a medium containing said analyte of interest, the analyte binds to said receptive material in said active areas and subsequently facilitate diffraction of transmitted light or reflected light in a diffraction pattern corresponding to said active areas.
3 more elements
6 The biosensor as in claim 5, wherein said receptive material is an antibody.
A method of making a biosensor, comprising the steps of:
- incorporating a photo-reactive agent at a surface of a substrate member;
- forming a receptive material layer over the photo-reactive agent generally uniformly over the surface of the substrate member, the layer containing a receptive material specific for an analyte of interest;
- placing a mask over the substrate member, the mask having a configuration so as to shield at least one underlying area of the substrate member while exposing at least one adjacent area;
- irradiating the substrate member and mask combination with an energy source sufficient to activate the photo-reactive agent underlying the receptive material layer in the areas exposed by the mask, the activated photo-reactive agent rendering the receptive material inactive in the exposed areas;
- removing the mask from the substrate member;
- and
- wherein a resulting pattern of active and inactive areas of the receptive material are defined, the inactive areas corresponding to the areas exposed by the mask and the active areas corresponding to the areas shielded by the mask such that when the biosensor is exposed to a medium containing the analyte of interest, the analyte binds to the receptive material in the active areas and subsequently facilitates diffraction of transmitted or reflected light in a diffraction pattern corresponding to the active areas.
4 more elements
Dependencies are inferred from the claim text.