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AssigneePharmacia Corporation
InventorsCarolyn J. Sympson, Rajeev Aurora, Stanton B. Dotson, Ronald B. Frazier, Cynthia L. Woods, Hamideh Zakeri, Xianzhi Zhou
Estimating the term…
Methionine aminopeptidases catalyse the co-translational removal of amino terminal methionine residues from nascent polypeptide chains. A newly-discovered enzyme, designated methionine aminopeptidase type-3 (MetAP-3), has a substrate specificity which is similar to MetAP-1 and MetAP-2, although it is not inhibited by fumagillin, an irreversible inhibitor of MetAP-2. MetAP-3 also preferentially localizes to mitochondria, unlike MetAP-1 and MetAP-2, which accumulate in the cytoplasm. One embodiment of the present invention relates to human cDNAs encoding polypeptides comprising MetAP-3. Other embodiments of the invention relate to nucleic acid molecules derived from these cDNAs, including complements, homologues, and fragments thereof, and methods of using these nucleic acid molecules, to generate polypeptides and fragments thereof. Other embodiments of the invention relate to antibodies directed against polypeptides comprising MetAP-3, and methods to screen for compounds or compositions that preferentially or specifically effect the activity of polypeptides comprising MetAP-3.
A purified and isolated polypeptide selected from the group consisting of:
A recombinant polypeptide selected from the group consisting of:
An isolated and purified antibody having a binding specificity for a polypeptide selected from the group consisting of:
A method for detecting a polypeptide in a biological fluid, wherein said polypeptide is selected from the group consisting of:
A method for detecting a first polypeptide in a biological fluid, wherein said first polypeptide is selected from the group consisting of:
An isolated nucleic acid molecule selected from the group consisting of:
An isolated nucleic acid molecule encoding MetAP-3.
An isolated nucleic acid molecule encoding a polypeptide comprising MetAP-3.
An isolated nucleic acid molecule encoding a polypeptide having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a polypeptide comprising the amino acids set forth in SEQ ID NO:
An isolated nucleic acid molecule comprising the nucleotides set forth in SEQ ID NO:
An isolated nucleic acid molecule comprising the nucleotides set forth SEQ ID NO:
An isolated nucleic acid molecule comprising the nucleotides set forth SEQ ID NO:
An isolated nucleic acid comprising nucleotides 10 through 30 of SEQ ID NO:
An isolated nucleic acid comprising nucleotides 10 through 30 of SEQ ID NO:
An isolated nucleic acid comprising nucleotides 13 through 30 of SEQ ID NO:
An isolated nucleic acid comprising nucleotides 13 through 30 of SEQ ID NO:
A composition consisting essentially of a buffer and a polypeptide selected from the group consisting of:
A method for determining the presence of nucleic acid encoding MetAP-3 in a cell comprising:
A method of removing an N-terminal methionine from a recombinant protein which comprises contacting said recombinant protein with MetAP-3 such that said N-terminal methionine is removed and recovering the resulting recombinant protein.
Dependencies are inferred from the claim text.