Nucleic acid fragments for the identification of bacteria in industrial wastewater bioreactors
AssigneeE. I. Du Pont de Nemours and Company
InventorsMichael G. Bramucci, Mario W. Chen, Helene Marie Albertson-Kane, Vasantha Nagarajan
Life of this patent
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Abstract
Unique bacterial strains and identifying 16S rDNA sequences have been isolated from activated wastewater sludge. The 16S rDNA sequences are diagnostic for organisms which are key to the health of activated sludge wastewater systems.
Claims15
An isolated 16S rDNA sequence indicative of the presence of an activated sludge bacterial strain selected from the group consisting of:
- (a) SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6 and SEQ ID NO:
- 7;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 6×SSC (1 M NaCl), 50% formamide, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C.
- (c) an isolated nucleic acid molecule that has at least 97% identity to (a);
- and
- (d) an isolated nucleic acid molecule that is completely complementary to (a), (b) or (c).
An isolated 16S rDNA sequence indicative of an activated sludge bacterial strain from the group consisting of:
- (a) SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, and SEQ ID NO:
- 30;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 6×SSC (1 M NaCl), 50% formamide, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C.
- (c) an isolated nucleic acid molecule that has at least 97% identity to (a);
- and
- (d) an isolated nucleic acid molecule that is completely complementary to (a), (b) or (c).
An isolated 16S rDNA sequence indicative of an activated sludge bacterial strain selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7;
- SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, and SEQ ID NO:
- 30.
A pyruvate degrading bacterial strain having the 16S rDNA sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 5, SEQ ID NO:
- 6, and SEQ ID NO:
- 7.
An acetate degrading bacterial strain having the 16S rDNA sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO:
- 4.
A benzoate degrading bacterial strain having the 16S rDNA sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 6, and SEQ ID NO:
- 7.
A denitrifying bacterial strain having the 16S rDNA sequence selected from the group consisting of SEQ ID NO:
- 1 and SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.