DNA authentication
OwnerOlivier Pelletierinventor
Life of this patent
Estimating the term…
Loading patent…
OwnerOlivier Pelletierinventor
Estimating the term…
The invention concerns a method and device for comparing the sequence of test DNA molecule contained in a first solution with the sequence of a reference comprising the steps of extracting (11) single-stranded DNA molecules from the first solution; mixing (14) a part of the solution containing the test DNA molecules with reference DNA molecules (13) attached to a solid support and having a sequence complementary to that of the sequence of reference; filtering (15) the mixture with a filter having a cut-off size chosen not to retain eventual free test DNA molecules but to retain the solid support and the test DNA molecules attached to it; introducing a solvent (16) in the filtered solution; illuminating (17) the final mixture; and measuring (18) the opacity or turbidity of the illuminated mixture, a non transparency indicating a non identity of the test and reference DNA sequences.
A method for determining whether or not the sequence of a test DNA molecule and the sequence of a reference DNA molecule are 95 to 100% identical, comprising the steps of:
A device (20) for comparing a test DNA sequence with a reference DNA sequence, comprising:
The device of claim 8, also comprising a photodiode unit (31, 32) to record the light intensity scattered by the indicator solution in detection chamber (26). 10. The device of any of claims 7 to 9, in which the detection chamber further comprises a first compartment (26) to contain the indicator solution outputted from the hybridization chamber (23), and a second compartment (22) to contain a control solution of the DNA solution to be tested.
Dependencies are inferred from the claim text.