Novel acidic mammalian proteins and polynucleotides encoding the same
AssigneeGenentech, Inc.
InventorsKaren M. Kelly, David A. Lewin, Timothy A. Stewart
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Abstract
Genes are disclosed that are differentially-regulated during feeding and fasting cycles. These genes, and their encoded polypeptides are useful to combat obesity and other metabolic disorders.
Claims47
An isolated polypeptide, having at least 80% sequence identity to the sequence of SEQ ID NO:2.
An isolated polynucleotide, having at least 80% sequence identity to the sequence of SEQ ID NO:1, or a complement of said polynucleotide.
A method of treating a metabolic disorder, comprising modulating the activity of Acidic Mammalian Molecule.
A method of detecting a disorder associated with changes in Acidic Mammalian Molecule gene expression, comprising:
- detecting a change in expression or activity of Acidic Mammalian Molecule.
A method for determining whether a compound up-regulates or down-regulates the transcription of an Acidic Mammalian Molecule gene, comprising:
- contacting said compound with a composition comprising a RNA polymerase and said gene and measuring the amount of Acidic Mammalian Molecule gene transcription.
A method for determining whether a compound up-regulates or down-regulates the translation of an Acidic Mammalian Molecule gene, comprising:
- contacting said compound with a composition comprising a ribosome and a polynucleotide corresponding to a mRNA of said gene and measuring the amount of Acidic Mammalian Molecule gene translation.
A transgenic non-human animal, having a disrupted Acidic Mammalian Molecule gene.
A transgenic non-human animal, comprising an exogenous polynucleotide having at least 80% sequence identity to the sequence of SEQ ID NO:1, or a complement of said polynucleotide.
A method of screening a sample for an Acidic Mammalian Molecule gene mutation, comprising:
- comparing an Acidic Mammalian Molecule nucleotide sequence in the sample with SEQ ID NO:1.
A method of measuring Acidic Mammalian Molecule agonist or antagonist activity of a compound comprising:
- contacting the compound with a composition comprising a polypeptide having at least 80% sequence identity to the sequence of SEQ ID NO:2 and,
- determining if Acidic Mammalian Molecule activity is changed.
Dependencies are inferred from the claim text.