Fluorobodies: binding ligands with intrinsic fluorescence
AssigneesLos Alamos National Laboratory, The Regents of the University of California
InventorsAndrew M. Bradbury, Ahmet Zeytun, Geoffrey S. Waldo
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Abstract
The current invention provides binding ligands with intrinsic fluorescent activity. The invention also provides libraries of such binding ligands, methods of preparing such binding ligands and libraries, and methods of identifying a binding ligand that specifically binds to a target molecule.
Claims37
A binding ligand with intrinsic fluorescence comprising a fluorescent protein that has a structure with a root mean square deviation of less than 5 angstroms from the 11 strand beta barrel structure of the green fluorescent protein (GFP) structure MMDB Id:
- 5742;
- wherein the fluorescent protein comprises heterologous binding sites in at least two loop positions on the surface of the fluorescent protein;
- and has fluorescent activity.
A method of preparing a binding ligand with intrinsic fluorescence that binds to a target antigen, the method comprising providing a fluorescent protein that has a structure with a root mean square deviation of less than 5 angstroms from the 11 strand beta barrel structure of the green fluorescent protein (GFP) structure MMDB Id:
- 5742;
- and inserting a heterologous binding site into at least two loop regions on the surface of the protein, thereby obtaining a binding ligand with intrinsic fluorescence.
Dependencies are inferred from the claim text.