Geminivirus resistant transgenic plants
OwnerLinda Hanley-Bowdoin, Sharon Settlageinventors
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Abstract
Transgenic plants with increased resistance to geminivirus infection, and nucleic acid constructs useful in producing such plants, are described. The transgenic plants express a mutant AL3/C3 geminivirus protein, which increases resistance to infection by at least one geminivirus, compared to a non-transformed control plant.
Claims18
A plant comprising transformed plant cells, said transformed plant cells containing a heterologous nucleic acid construct comprising, in the 5′ to 3′ direction:
- (a) a promoter operable in said plant cells,
- (b) a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and
- (c) a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith,
- wherein expression of said mutant AL3/C3 protein increases resistance of said plant to infection by at least one geminivirus, compared to a non-transformed control.
A tomato plant comprising transformed tomato plant cells, said transformed tomato plant cells containing a heterologous nucleic acid construct comprising, in the 5′ to 3′ direction:
- (a) a promoter operable in said plant cells,
- (b) a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and
- (c) a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith,
- wherein expression of said mutant AL3/C3 protein increases resistance of said tomato plant to infection by at least one geminivirus, compared to a non-transformed control.
A plant of the family Solanaceae, wherein cells of said plant are transformed to contain a heterologous nucleic acid construct comprising, in the 5′ to 3′ direction:
- (a) a promoter operable in said plant cells,
- (b) a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and
- (c) a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith,
- wherein expression of said mutant AL3/C3 protein increases resistance of said plant to infection by at least one geminivirus, compared to a non-transformed control.
A method of combating geminivirus infection in an agricultural field, comprising planting the field with a crop of plants comprising transformed plant cells, said transformed plant cells containing a heterologous nucleic acid construct comprising, in the 5′ to 3′ direction:
- (a) a promoter operable in said plant cells,
- (b) a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and
- (c) a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith,
- wherein expression of said mutant AL3/C3 protein increases resistance of said plants to infection by at least one geminivirus, compared to a non-transformed control.
A method of making a transgenic plant having increased resistance to geminivirus infection, said method comprising:
- providing a plant cell capable of regeneration;
- transforming said plant cell with a DNA construct comprising, in the 5′ to 3′ direction, a promoter operable in said plant cell, a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith;
- and then
- regenerating a transgenic geminivirus-resistant plant from said transformed plant cell, wherein expression of said mutant AL3/C3 protein increases resistance of said plant to infection by at least one geminivirus, compared to a non-transformed control.
A nucleic acid construct comprising an expression cassette, which construct comprises, in the 5′ to 3′ direction:
- (a) a promoter operable in a plant cell,
- (b) a nucleic acid sequence encoding a mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and
- (c) a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith.
A method of producing nucleic acid constructs useful in conferring increased geminivirus-resistance to plants, comprising:
- a) identifying mutants of a geminivirus AL3/C3 protein that enhance geminivirus resistance in plant cells when expressed therein;
- b) preparing a nucleic acid construct comprising, in the 5′ to 3′ direction, a promoter operable in a plant cell, a nucleic acid sequence encoding said mutant AL3/C3 protein, said nucleic acid sequence located downstream from said promoter and operatively associated therewith, and a termination sequence positioned downstream from said nucleic acid sequence and operatively associated therewith.
Dependencies are inferred from the claim text.