Isolated nucleic acid molecules which encode peptides that bind to HLA molecules, and uses thereof
AssigneeLudwig Institute for Cancer Research
InventorsDanila Valmori, Jean-Charles Cerottini, Pedro Romero
Life of this patent
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Abstract
Nonapeptides and decapeptides which bind to HLA molecules and provoke proliferation of cytolytic T cells are disclosed. The decapeptides terminate in Valine, and are restricted in their first three amino acid positions. Other useful nonapeptides are also disclosed.
Claims18
An isolated peptide which binds to an HLA-A2 molecule, said peptide consisting of ten amino acids, wherein the carboxy terminal amino acid is Val, the amino terminal amino acid is Ala, Tyr or Phe, and the second amino acid is Ala.
An isolated peptide which binds to an HLA-A2 molecule and consists often amino acids, said isolated peptide having Val at its carboxy terminus, Glu at its amino terminus, and the second and third amino acids from the N-terminus are Ala, Leu, or Met, with the proviso that when the second amino acid is Ala, the third amino acid must be Leu or Met, and when the third amino acid is Ala, the second amino acid must be Leu or Met.
Isolated nonapeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, and SEQ ID NO:
- 8.
Isolated decapeptide consisting of the amino acid sequence set forth in SEQ ID NO:
- 3, SEQ ID NO:
- 23, or SEQ ID NO:
- 24.
Isolated decapeptide which has an amino acid sequence selected from the group consisting of SEQ ID NO:
- 13, SEQ ID NO:
- 14, and SEQ ID NO:
- 16.
A method for provoking proliferation of a cytolytic T lymphocyte which is reactive with a cell that presents, on its surface, a complex of an HLA-A2 molecule and a peptide which forms a non-covalent complex with said HLA-A2 molecule comprising contacting a sample containing said cytolytic T lymphocyte with a complex of an HLA-A2 molecule and a nonamer or decamer, wherein the carboxy terminal amino acid for said nonamer or decamer is Val, the amino terminal amino acid for said nonamer or decamer is Ala, Glu, Tyr, or Phe, and the second amino acid of said nonamer or decamer is Ala or Leu, so as to stimulate proliferation of cytolytic T cells which react with complexes of HLA-A2 and said nonamer or decamer, and at least one other complex of HLA-A2 and a second, different nonamer or decamer.
Dependencies are inferred from the claim text.