Disposable electrode for whole blood hemoglobin (HGB) and hematocrit (HCT) measurement, and preparation and application

A process for producing disposable hemoglobin and hematocrit detecting electrode strip, which comprises:

• (a) coating a conducting film on one side of an electric insulating substrate and forming isolated and disconnected an anode and a cathode;
• (b) coating an electric insulating film on a part of the conducting film, wherein one end of an uncovered anode of the conducting film is at least a reference electrode and the other end an anode connector, and one end of an uncovered cathode of the conducting film is at least a working electrode and the other end a cathode connector;
• and
• (c) attaching a reaction film to an electric insulating substrate, wherein the reaction film cover a region containing at least the working electrode and the reference electrode so as to connect the working electrode and the reference electrode individually, and wherein the reaction film comprises a carrier, a surfactant and a conductive mediator, the carrier comprises a microcrystalline cellulose, a polymer and a buffer solution;
• the surfactant comprises an amphoteric substance having a hydrophobic moiety and a hydrophilic moiety;
• and the conductive mediator comprises an electrolyte with a higher redox potential than that of methemoglobin.

A hemoglobin and hematocrit detecting equipment, which comprises:

• (A) a disposable hemoglobin and hematocrit detecting electrode strip, said strip comprises:
• (a) an electric insulating substrate;
• (b) a conducting film coated on one side of the electric insulating substrate to form isolated and disconnected an anode and a cathode;
• (c) an electric insulating film coated on a part of the conducting film, wherein one end of an uncovered anode of the conducting film forms at least a reference electrode and the other end an anode connector, and one end of an uncovered cathode of the conducting film forms at least a working electrode and the other end a cathode connector;
• and
• (d) a reaction film comprising a carrier, a surfactant and a conductive mediator, being screen printed on a region containing at least the working electrode and the reference electrode so as to connect the working electrode and the reference electrode individually, wherein the carrier comprises a microcrystalline cellulose, a polymer and a buffer solution;
• the surfactant comprises an amphoteric substance having a hydrophobic moiety and a hydrophilic moiety;
• and the conductive mediator comprises an electrolyte with a higher redox potential than that of methemoglobin;
• and
• (B) an amperometric sensor, which comprises a voltage output equipment, a signal receiver and a display equipment;
• wherein the amperometric sensor is connected to the anode connector and the cathode connector of the hemoglobin and hematocrit detecting electrode strip;
• the voltage output equipment provides a voltage of below 400 mV to the reaction film of the hemoglobin and hematocrit detecting electrode strip so as to oxidize the conductive mediator from reduction state to oxidation state after being reacted with hemoglobin and hematocrit of the sample;
• the signal receiver receives a current, voltage or resistance change generated during a redox reaction and transmits the change to the display equipment to display the concentration of hemoglobin and the hematocrit value in the sample.