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US 2003/0079255 A1Published application
Methods for the production of tyrosine, cinnamic acid and para-hydroxycinnamic acid
AssigneeE. I. Du Pont de Nemours and Company
InventorsWei Wei Qi, Fateme Sima Sariaslani, Xiao-Song Tang
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Abstract
Genes encoding phenylalanine ammonia-lyase (PAL), tyrosine ammonia lyase (TAL) and phenylalanine hydroxylase (PAH) have been introduced into a host organism for the production of Para-hydroxycinnamic acid (PHCA). The introduction of these genes results in the redirection of carbon flow in the host, optimizing the flow of carbon from glucose to PHCA. The intermediates, tyrosine and cinnamic acid are also produced.
Claims12
A method for the production of para-hydroxycinnamic acid comprising:
- a) providing a recombinant organism comprising:
- i) at least one gene encoding a tyrosine ammonium lyase activity;
- and
- ii) at least one gene encoding a phenylalanine hydroxylase activity;
- b) growing said recombinant organism in the presence of a fermentable carbon substrate whereby para-hydroxycinnamic is produced.
2 more elements
A method for the production of tyrosine comprising:
- a) providing a recombinant organism comprising at least one gene encoding a phenylalanine hydroxylase activity;
- and
- b) growing said recombinant organism in the presence of a fermentable carbon substrate whereby tyrosine is produced.
A recombinant host comprising:
- i) at least one gene encoding a tyrosine ammonium lyase activity;
- and
- ii) at least one gene encoding a phenylalanine hydroxylase activity.
Dependencies are inferred from the claim text.