Compositions and methods for recombinational cloning of nucleic acid molecules

A method for cloning at least one nucleic acid molecule comprising a nucleic acid segment flanked by at least two recombination sites, wherein the recombination sites do not substantially recombine with each other, the method comprising:

• (a) forming a combination by combining in vitro or in vivo:
• (i) at least one Insert Donor molecule comprising the nucleic acid molecule;
• (ii) at least one first Vector Donor molecule comprising at least two recombination sites, wherein the recombination sites do not substantially recombine with each other;
• (iii) an effective amount of at least one recombination protein;
• and
• (iv) an effective amount of at least one Fis protein or Fis protein fragment;
• and
• (b) incubating the combination under conditions sufficient to transfer the nucleic acid molecule into the first Vector Donor molecule, thereby producing at least one first Product molecule.