Cytochrome P450 oxygenases and their uses
AssigneeWashington State University Research Foundation
InventorsRodney B. Croteau, Anne Schoendorf, Stefan Jennewein
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Abstract
Oxygenase enzymes and the use of such enzymes to produce (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
Claims40
A purified protein, comprising an amino acid sequence selected from the group consisting of:
- SEQ ID NOS:
- 22-42, 56-86, and 87-92.
An isolated nucleic acid molecule that:
- (a) hybridizes under low-stringency conditions with a nucleic acid probe, the probe comprising a sequence selected from the group consisting of SEQ ID NOS:
- 1-21, 43-55, and 81-86 and fragments thereof;
- and
- (b) encodes a protein having oxygenase activity.
An isolated nucleic acid molecule that:
- (a) has at least 60% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 1-21, 56-68, and 81-86;
- and
- (b) encodes a protein having oxygenase activity.
A method for isolating a nucleic acid sequence, comprising:
- (a) hybridizing the nucleic acid sequence to at least 10 contiguous nucleotides of a sequence selected from the group consisting of SEQ ID NOS:
- 1-21, 56-68, and 81-86;
- and
- (b) identifying the nucleic acid sequence as one that encodes an oxygenase.
A purified protein having oxygenase activity, comprising an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 56-68 and 87-92;
- (b) an amino acid sequence that differs from the amino acid sequence specified in (a) by one or more conservative amino acid substitutions;
- and
- (c) an amino acid sequence having at least 70% sequence identity to the sequences specified in (a) or (b).
Dependencies are inferred from the claim text.