LDL receptor signaling assays
AssigneeBoard of Regents, The University of Texas System
InventorsJoachim Herz, Petra May
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Abstract
The invention provides methods and compositions for modeling and detecting LDL receptor transmembrane signaling by detecting proteolysis of an LDL receptor transmembrane domain. The method comprises the steps of: a) providing a sample comprising a cell membrane comprising (i) a polypeptide comprising an LDL receptor transmembrane domain fused to a C-terminal tail, and (ii) a protease which specifically cleaves the domain and thereby releases the tail from the membrane; b) incubating the sample under conditions wherein the protease cleaves the domain and thereby releases the tail from the membrane; and c) detecting a resultant released tail.
Claims20
A method for detecting proteolysis of an LDL receptor transmembrane domain, comprising the steps of:
- a) providing a sample comprising a cell membrane comprising (i) a polypeptide comprising an LDL receptor transmembrane domain fused to a C-terminal tail, and (ii) a protease which specifically cleaves the domain and thereby releases the tail from the membrane;
- b) incubating the sample under conditions wherein the protease cleaves the domain and thereby releases the tail from the membrane;
- and
- c) detecting a resultant released tail.
Dependencies are inferred from the claim text.