Methods and compounds for disruption of CD40R/CD40L signaling in the treatment of alzheimer's disease
OwnerJun Tan, Terrence C. Town +1inventors
InventorsJun Tan, Terrence C. Town, Michael Mullan
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Abstract
The subject invention provides methods of treating amyloidogenic diseases, comprising the administration of therapeutically effective amounts of a composition comprising a carrier and an agent that interferes with the interaction of CD40L and CD40R to an individual afflicted with an amyloidogenic disease. Also provided are methods and/or assay systems for the identification of compounds or other small molecules capable of disrupting the CD40R/CD40L signaling pathway.
Claims25
A method of identifying compounds that modulate the CD40 ligand/CD40 receptor (CD40L/CD40R) signaling pathway comprising contacting a first sample of cells expressing CD40 receptor (CD40R) with CD40 ligand (CD40L) and measuring a marker;
- contacting a second sample of cells expressing CD40R with a compound and CD40 ligand, and measuring said marker;
- and comparing said marker of said first sample of cells with said marker of said second sample of cells.
A method of identifying compounds that modulate the CD40L/CD40R signaling pathway comprising:
- a. contacting CNS cells expressing CD40R with CD40 ligand and a compound and measuring a marker;
- b. contacting peripheral cells expressing CD40R with CD40 ligand and said compound and measuring a marker;
- c. contacting CNS cells with a stimulator of the CD40L/CD40R signaling pathway and a compound and measuring a marker;
- d. contacting peripheral cells with a stimulator of the CD40L/CD40R signaling pathway and said compound and measuring a marker;
- e. contacting CNS cells with an inhibitor of the CD40L/CD40R signaling pathway and said compound and measuring a marker;
- f. contacting peripheral cells with an inhibitor of the CD40L/CD40R signaling pathway and said compound and a marker;
- and
- g. comparing said markers to identify those compounds that modulate the CD40L/CD40R signaling pathway.
A method of identifying compounds that reduce, ameliorate, or modulate symptoms associated with neuronal inflammation, brain injury/trauma, tauopathies, or amyloidogenic diseases comprising administering a compound that modulates the CD40L/CD40R signaling pathway to an animal model and measuring or observing the reduction, amelioration, or modulation of said symptoms.
A method of treating neuronal inflammation, brain injury/trauma, tauopathies, or amyloidogenic diseases comprising the administration, to an individual, of therapeutically effective amounts of a composition comprising a carrier and an agent that interferes with CD40L/CD40R signaling pathway or the phosphorylation of tau protein.
Dependencies are inferred from the claim text.