Non-polar media for polynucleotide separations
OwnerDouglas T. Gjerde, Paul D. Taylorinventors
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Abstract
Nonporous beads having an average diameter of about 0.5-100 microns are suitable for chromatographic separation of mixtures of polynucleotides when the beads comprise a nonporous particle which are coated with a polymer or which have substantially all surface substrate groups endcapped with a non-polar hydrocarbon or substituted hydrocarbon group. The beads provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography.
Claims41
A method for separating a mixture of polynucleotides, comprising applying a mixture of polynucleotides having up to 1500 base pairs to a separation medium, the separation surfaces of said medium coated with a hydrocarbon or non-polar hydrocarbon substituted polymer, or having substantially all polar groups reacted with a non-polar hydrocarbon or substituted hydrocarbon group, wherein said surfaces are non-polar;
- and eluting said mixture of polynucleotides.
A bead comprising a nonporous particle coated with a polymer, wherein said bead has an average diameter of 0.5 to 100 microns and wherein said bead is characterized by having a Mutation Separation Factor of at least 0.1.
A bead comprising a nonporous particle having substantially all surface substrate groups endcapped with a non-polar hydrocarbon or substituted hydrocarbon group, wherein said bead has an average diameter of 0.5 to 100 microns and wherein said bead is characterized by having a Mutation Separation Factor of at least 0.1 using Matched Ion Polynucleotide Chromatography.
A silica gel monolith having non-polar interstitial separation surfaces wherein said monolith has been subjected to an acid wash treatment in order to substantially remove multivalent cation contaminants from said surfaces.
Dependencies are inferred from the claim text.