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OwnerWilliam D. Hitz, Scott Anthony Sebastianinventors
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A soybean enzyme, myo-inositol 1-phosphate synthase, whose manipulation results in the alteration of raffinose saccharide, sucrose, phytic acid and inorganic phosphate content of soybean seeds, thus leading to valuable and useful soybean products, has been identifed. Soybean lines with decreased capacity for the synthesis of myo-inositol 1-phosphate in the tissue of developing seeds in comparison to seeds of other soybean lines, have been developed. As taught herein, reduction of myo-inositol 1-phosphate synthase enzymatic activity by any of several means will result in soybean seeds displaying this desirable phenotype.
An isolated nucleic acid fragment encoding a soybean myo-inositol 1-phosphate synthase.
An isolated nucleic acid fragment encoding a mutant myo-inositol 1-phosphate synthase having decreased capacity for the synthesis of myo-inositol-1-phospate.
A soybean plant with a heritable phenotype of (i) a seed phytic acid content of less than 17 μmol/g, (ii) a seed content of raffinose plus stachyose of less than 14.5 μmol/g, and (iii) a seed sucrose content of greater than 200 μmol/g, provided that the plant is not LR33.
A soy protein product derived from seeds of a soybean plant homozygous for at least one gene encoding a mutant myo-inositol 1-phosphate synthase having decreased capacity for the synthesis of myo-inositol 1-phosphate, the gene conferring a heritable phenotype of (i) a seed phytic acid content less than 17 μmol/g, (ii) a seed content of raffinose plus stachyose of less than 14.5 μmol/g, and (iii) a seed sucrose content of greater than 200 μmol/g.
A method of using a soybean plant homozygous for at least one gene encoding a mutant myo-inositol 1-phosphate synthase having decreased capacity for the synthesis of myo-inositol 1-phosphate, the gene conferring a heritable phenotype of (i) a seed phytic acid content less than 17 μmol/g, (ii) a seed content of raffinose plus stachyose of less than 14.5 μmol/g, and (iii) a seed sucrose content of greater than 200 μmol/g to produce progeny lines, the method comprising:
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