Administration of nucleic acid sequence to female animal to enhance growth in offspring
OwnerRobert J. Schwartz, Ruxandra Draghia-Akli +3inventors
InventorsRobert J. Schwartz, Ruxandra Draghia-Akli, Roy G. Smith, Douglas R. Kern, Robert H. Carpenter
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Abstract
Growth is improved by utilizing growth enhancement potential methodology to administer a nucleic acid sequence, such as GHRH or an analog, to a female animal, preferably through a parenteral route of administration. Piglets born from sows injected with DNA encoding GHRH are larger, and effects are demonstrated in subsequent pregnancies without additional administration(s) of the vector.
Claims136
A method of improving or enhancing growth in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in improved or enhanced growth in said offspring.
A method of increasing levels of growth hormone in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased levels of growth hormone in said offspring.
A method of increasing lean body mass in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased lean body mass in said offspring.
A method of increasing levels of IGF-I in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased levels of IGF-I in said offspring.
A method of increasing feed efficiency in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased feed efficiency in said offspring.
A method of increasing the rate of growth in an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased rate of growth in said offspring.
A method of increasing the ratio of somatotrophs to other hormone-producing cells in a pituitary gland of an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in ratio of somatotrophs to other hormone-producing cells in a pituitary gland in said offspring.
A method for delaying birth of an offspring from a female animal comprising the step of introducing an effective amount of a vector into cells of said female animal prior to or during gestation of said offspring, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in delayed birth in said offspring.
A method of increasing milk production in an animal comprising the step of introducing an effective amount of a vector into cells of said animal, wherein said vector comprises a promoter;
- a nucleotide sequence;
- and a 3′ untranslated region, under conditions wherein the nucleotide sequence is expressed and wherein said introduction and expression of said vector results in increased milk production in said animal.
Dependencies are inferred from the claim text.