Improved materials and methods for transformation
OwnerLada Rasochova, Thomas German +1inventors
InventorsLADA RASOCHOVA, THOMAS GERMAN, PAUL AHLQUIST
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Abstract
Disclosed herein are novel methods and materials directed to transforming a host cell and expressing exogenous RNA therein. Specifically disclosed are DNA-launching platforms used to introduce a replicating viral segment attached to an exogenous polynucleotide into a cell, whereby the exogenous polynucleotide is expressed in said cell and confers a detectable trait.
Claims30
A DNA-launching platform comprising:
- a) a polynucleotide molecule encoding a modified viral RNA molecule;
- and
- b) a DNA dependent RNA polymerase promoter.
A method of genotypically or phenotypically modifying one or more cells comprising the following steps:
- a) obtaining a DNA-launching platform comprising a polynucleotide molecule encoding a modified viral RNA;
- and
- b) transfecting said one or more cells with said DNA-launching platform, wherein said polynucleotide molecule is transcribed thereby forming a replicatable RNA transcript.
A method of producing a plant or plant tissue comprising at least one genotypically or phenotypically modified cell, said method comprising transfecting cells of said plant or plant tissue with a DNA-launching platform, wherein said DNA-launching platform comprises a polynucleotide encoding a modified RNA molecule, such that said polynucleotide molecule is transcribed to form a replicatable RNA transcript.
Dependencies are inferred from the claim text.