Method for transferring nucleic acid into striated muscles
AssigneeAventis Pharma S.A.
InventorsMichel Bureau, Lluis Mir, Daniel Scherman
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Abstract
The invention provides a method of transferring in vivo a molecule into a striated muscle cell. More specifically, a method of the invention comprises contacting in vivo a striated muscle cell with a molecule, and electrically stimulating the muscle cell with one or more unipolar pulses of an electric field intensity ranging from 1 to 800 V/cm2. In one embodiment, the molecule is a nucleic acid encoding a protein of interest. For example, the invention provides methods of promoting angiogenesis and hemostasis.
Claims81
Method of transferring nucleic acid into one or more striated muscles in vivo in which the muscle cells are brought into contact with the nucleic acid to be transferred by direct administration into the tissue or by topical or systemic administration and in which the transfer is brought about by application to the said muscle of one or more electrical pulses of an intensity of between 1 and 800 volts/cm.
Nucleic acid and electric field of an intensity between 1 and 800 volts/cm, as combination product for their administration simultaneously, separately or spaced out over time in vivo to the striated muscle and, for gene therapy based on in vivo electrotransfection into the striated muscle.
Dependencies are inferred from the claim text.