Omi and domains thereof that disrupt IAP-caspase interaction
OwnerEmad S. Alnemriinventor
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Abstract
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a peptide or polypeptide of Omi having at least an N-terminus amino acid sequence of Ala-Val-Pro-Ser and up to 321 contiguous amino acid residues that can be derived from residues 138-458 of SEQ ID NO:1 or a functional variant of each, each of which is capable of specifically binding to at least a portion of an Inhibitor of Apoptosis protein. This peptide can be used in a method to modulate apoptosis or to identify modulators of apoptosis as well as in therapeutic uses.
Claims75
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a peptide or polypeptide of Omi comprising SEQ ID NO:3 and up to 321 contiguous amino acid residues derived from residues 138-458 of SEQ ID NO:1 that specifically binds to a portion of an Inhibitor of Apoptosis Protein (IAP).
An isolated peptide or polypeptide comprising SEQ ID NO:3 and up to 321 contiguous amino acid residues derived from residues 138-458 of SEQ ID NO:1 that specifically binds to a portion of an Inhibitor of Apoptosis Protein (IAP).
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a polypeptide of Omi comprising SEQ ID NO:11 and up to 314 additional contiguous amino acid residues derived from residues 138-458 of SEQ ID NO:1, wherein said polypeptide induces caspase-independent apoptosis.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a polypeptide of Omi comprising SEQ ID NO:12, SEQ ID NO:11, and up to 321 contiguous amino acid residues including SEQ ID NO:11 derived from residues 138-458 of SEQ ID NO:1, wherein said polypeptide induces caspase-independent apoptosis.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a polypeptide of Omi comprising SEQ ID NO:3, SEQ ID NO:13, and up to 321 contiguous amino acid residues including SEQ ID NO:13 derived from residues 138-458 of SEQ ID NO:1, wherein said polypeptide fails to have serine protease activity.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence encoding a polypeptide of Omi comprising the amino acid sequence of residues 134-458 of SEQ ID NO:1 except that the serine at position 306 is mutated and said polypeptide fails to have serine protease activity.
An isolated polypeptide of Omi comprising SEQ ID NO:11 and up to 314 additional contiguous amino acid residues derived from residues 138-458 of SEQ ID NO:1 wherein said polypeptide induces caspase-independent apoptosis.
An isolated polypeptide of Omi comprising SEQ ID NO:12, SEQ ID NO:11, and up to 321 contiguous amino acid residues including SEQ ID NO:11 derived from residues 138-458 of SEQ ID NO:1, wherein said polypeptide induces caspase-independent apoptosis.
An isolated polypeptide of Omi comprising SEQ ID NO:3, SEQ ID NO:13, and up to 321 contiguous amino acid residues including SEQ ID NO:13 derived from residues 138-458 of SEQ ID NO:1, wherein said polypeptide fails to have serine protease activity.
An isolated polypeptide of Omi comprising the amino acid sequence of residues 134-458 of SEQ ID NO:1 except that the serine at position 306 is mutated and said polypeptide fails to have serine protease activity.
An antibody that specifically binds to an epitope located on the N-terminus of Omi.
Dependencies are inferred from the claim text.