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US 2003/0073086 A1Published application
Randomly ordered arrays and methods of making and using
AssigneeSurModics, Inc.
InventorsPatrick E. Guire, Kristin S. Taton, John V. Wall
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Abstract
Arrays including microparticles having probe and marker moieties are used for the detection of a target in a sample. Microparticles are randomly immobilized on at least a portion of a substrate. A detection scheme is performed to detect the marker associated with the microparticle and the identity of the probe, and any target bound to the probe.
Claims55
A method for detecting a target in a sample, the method comprising steps of:
- a) providing an array comprising
- (i) a substrate;
- and
- (ii) a plurality of microparticles randomly immobilized on the substrate via an immobilization material, wherein each microparticle comprises a self-encoding marker and a probe coupled to the microparticle, wherein each self-encoding marker and probe comprises a unique self-encoding marker/probe pair, and wherein the probe is configured and arranged to specifically bind the target;
- b) applying the sample suspected of containing the target to the array;
- c) maintaining the sample and array under conditions to allow binding of the target to the probe;
- and
- d) detecting the self-encoding marker coupled to the microparticle and a target marker associated with the microparticle.
5 more elements
A method for fabricating an array, the method comprising steps of:
- a) preparing a mixture having a plurality of microparticles, wherein each microparticle comprises a self-encoding marker and a probe coupled to the microparticle, wherein each self-encoding marker and probe comprises a unique self-encoding marker/probe pair, and wherein the probe is configured and arranged to specifically bind a target present in a sample;
- b) coating a substrate, and optionally the microparticle, with an immobilization material;
- c) disposing the mixture having a plurality of microparticles on the substrate, wherein the microparticles become immobilized in a random pattern on the substrate via the immobilization material.
An array comprising:
- a) a substrate;
- b) a plurality of microparticles randomly immobilized on the substrate, wherein each microparticle comprises a self-encoding marker and a probe coupled to the microparticle, wherein each self-encoding marker and probe comprises a unique self-encoding marker/probe pair, and wherein the probe is configured and arranged to specifically bind a target present in a sample;
- and
- c) an immobilization material which immobilizes the microparticles on the substrate.
1 more element
Dependencies are inferred from the claim text.