US 2003/0073071 A1 · Solid state sensing system and method for measuring the binding or hybridization of biomolecules

Abstract

Measurements relating to the activity of various molecules of interest are obtained using a sensing surface with an associated first charge, a charge-regulating layer bound to the sensing surface, various possible probe molecules and a measurement circuit. The sensing surface has a layer bound to it that confers a neutral charge or a second charge on a net basis. In addition, a probe is bound to at least one of the surface and the layer, the probe being complementary to and interacting with a molecule of interest. The interaction between the probe and the molecule of interest is detected electrically.

Claims46

Dependent claims

An apparatus for measuring biomolecular interaction, the apparatus comprising:

a. a sensing surface having an associated first charge;
b. a charge-regulating layer bound to the sensing surface, the charge-regulating layer having an associated second charge opposite to the first charge and presenting, in conjunction with the sensing surface, the second charge or a neutral charge on a net basis;
c. a probe bound to at least one of the sensing surface and the charge-regulating layer, the probe being complementary to and interacting with a molecule of interest;
and
d. a measurement circuit, operatively connected to the sensing surface, for measuring interactions between the probe and the molecule of interest.

2 more elements

Dependencies are inferred from the claim text.

Solid state sensing system and method for measuring the binding or hybridization of biomolecules

Life of this patent

Solid state sensing system and method for measuring the binding or hybridization of biomolecules

Life of this patent

Abstract

Claims46