Device for drug-drug interaction testing sample preparation

A device for the preparation of inhibitor compounds (NCEs) for testing of in vivo drug-drug interactions in mammals, wherein said device comprises a computer controlled and coordinated device having:a)

• a freezer unit for storing microsomes;
• b)thawing means for thawing said microsomes prior to use of the microsomes in the preparation of testing samples;
• c)timed computerized elements for removing frozen microsomes from the freezer unit to the thawing means for the thawing of the microsomes;
• d)two computer controlled and timed manipulation and transport mechanisms for the holding and transporting of multi-well test plates to operational stations of said device and for positioning of the plates at said operational stations for appropriate operation thereat;
• e)a first operation station with fluid introduction element for simultaneously filling each of a plurality of predetermined wells of a reaction test plate with a preset quantity of thawed microsomes;
• f)a second operation station for combining microsomes in respective wells of said reaction test plate with an inhibitor compound and a cytochrome P450 enzyme substrate;
• g)a third operation station for incubating said combination in each of the respective wells to an in vivo temperature for said mammal for reaction thereof for a pre-determined period of time to form reaction products;
• h)means for purification of any reaction products from the wells of said reaction plate;
• and
• i)computerized timing means for synchronizing simultaneous operations with respect to each of the plates and the respective contents in the wells thereof, and timing of the thawing and pre-incubation and incubation periods and initiation of thawing of microsomes for a subsequent test run;
• j)at least one holding area for each vial holding pre-thawed microsomes for use in subsequent test runs.