US 2003/0069404 A1Published application

Helicobacter antigens and corresponding DNA fragments

OwnerRainer Haas, Harold Kleanthous +4inventors

InventorsRainer Haas, Harold Kleanthous, Thomas F. Meyer, Stefan Odenbreit, Amal A. Al-Garawi, Charles A. Miller

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Abstract

The invention provides Helicobacter polypeptides that can be used in vaccination methods for preventing or treating Helicobacter infection, and polynucleotides that encode these polypeptides.

Claims39

Dependent claims

An isolated polynucleotide that encodes (i) a polypeptide comprising an amino acid sequence that is homologous to the amino acid sequence of a Helicobacter membrane-associated polypeptide, wherein said amino acid sequence of said Helicobacter membrane-associated polypeptide is selected from the group consisting of:

(a) the amino acid sequences as shown:
in SEQ ID NO:2, beginning with an amino acid in any one of the positions from −27 to 5, and ending with an amino acid in position 160 (HPO101);
in SEQ ID NO:4, beginning with an amino acid in position 1 and ending with an amino acid in position 172 (HPO104);
in SEQ ID NO:6, beginning with an amino acid in any one of the positions from −17 to 5, and ending with an amino acid in position 169 (HPO116);
in SEQ ID NO:8, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 198 (HPO121);
in SEQ ID NO:10, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 132 (HPO132);
in SEQ ID NO:12, beginning with an amino acid in positions 1 and ending with an amino acid in position 114 (HPO15);
in SEQ ID NO:14, beginning with an amino acid in any one of the positions from −17 to 5, and ending with an amino acid in position 248 (HPO18);
in SEQ ID NO:16, beginning with an amino acid in any one of the positions from −40 to 5, and ending with an amino acid in position 74 (HPO38);
in SEQ ID NO:18, beginning with an amino acid in any one of the positions from −34 to 5, and ending with an amino acid in position 226 (HPO42);
in SEQ ID NO:20, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 179 (HPO45);
in SEQ ID NO:22, beginning with an amino acid in any one of the positions from −33 to 5, and ending with an amino acid in position 114 (HPO50);
in SEQ ID NO:24, beginning with an amino acid in any one of the positions from −60 to 5, and ending with an amino acid in position 349 (HPO54);
in SEQ ID NO:26, beginning with an amino acid in any one of the positions from −18 to 5, and ending with an amino acid in position 288 (HPO57);
in SEQ ID NO:28, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 150 (HPO58);
in SEQ ID NO:30, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 309 (HPO64);
in SEQ ID NO:32, beginning with an amino acid in any one of the positions from −35 to 5, and ending with an amino acid in position 129 (HPO70);
in SEQ ID NO:34, beginning with an amino acid in any one of the positions from −19 to 5, and ending with an amino acid in position 153 (HPO71);
in SEQ ID NO:36, beginning with an amino acid in any one of the positions from −25 to 5, and ending with an amino acid in position 176 (HPO76);
in SEQ ID NO:38, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 156 (HPO7);
in SEQ ID NO:40, beginning with an amino acid in position 1 and ending with an amino acid in position 144 (HPO80);
in SEQ ID NO:42, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 152 (HPO87);
in SEQ ID NO:44, beginning with an amino acid in any one of the positions from −31 to 5, and ending with an amino acid in position 112 (HPO95);
in SEQ ID NO:46, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 91 (HPO98);
in SEQ ID NO:48, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 129 (HPO9);
and
(b) the precursor or mature amino acid sequences encoded by the Helicobacter DNA inserts found in American Type Culture Collection deposit numbers 98197 (HPO76), 98210 (HPO18), 98201 (HPO121), 98208 (HPO45), 98198 (HPO101), 98200 (HPO116), 98211 (HPO7), 98199 (HPO104), 98214 (HPO15), 98206 (HPO58), 98202 (HPO132), 98203 (HPO9), 98204 (HPO38), 98205 (HPO87), 98217 (HPO71), 98219 (HPO70), 98215 (HPO80), 98216 (HPO95), 98218 (HPO98), 98220 (HPO57), 98207 (HPO50), 98213 (HPO64), 98212 (HPO54), and 98209 (HPO42);
or (ii) a derivative of said polypeptide encoded by said polynucleotide.

25 more elements

An isolated polynucleotide that encodes (i) a polypeptide comprising an amino acid sequence that is homologous to an amino acid sequence selected from the group consisting of:

(a) the amino acid sequences as shown:
in SEQ ID NO:2, beginning with an amino acid in position −27 and ending with an amino acid in position 160 (HPO101);
in SEQ ID NO:4, beginning with an amino acid in position 1 and ending with an amino acid in position 172 (HPO104);
in SEQ ID NO:6, beginning with an amino acid in position −17 and ending with an amino acid in position 169 (HPO116);
in SEQ ID NO:8, beginning with an amino acid in position −21 and ending with an amino acid in position 198 (HPO121);
in SEQ ID NO:10, beginning with an amino acid in position −20, and ending with an amino acid in position 132 (HPO132);
in SEQ ID NO:12, beginning with an amino acid in position 1 and ending with an amino acid in position 114 (HPO15);
in SEQ ID NO:14, beginning with an amino acid in position −17 and ending with an amino acid in position 248 (HPO18);
in SEQ ID NO:16, beginning with an amino acid in position −40 and ending with an amino acid in position 74 (HPO38);
in SEQ ID NO:18, beginning with an amino acid in position −34 and ending with an amino acid in position 226 (HPO42);
in SEQ ID NO:20, beginning with an amino acid in position −21 and ending with an amino acid in position 179 (HPO45);
in SEQ ID NO:22, beginning with an amino acid in position −33 and ending with an amino acid in position 114 (HPO50);
in SEQ ID NO:24, beginning with an amino acid in position −60 and ending with an amino acid in position 349 (HPO54);
in SEQ ID NO:26, beginning with an amino acid in position −18 and ending with an amino acid in position 288 (HPO57);
in SEQ ID NO:28, beginning with an amino acid in position −21 and ending with an amino acid in position 150 (HPO58);
in SEQ ID NO:30, beginning with an amino acid in position −20 and ending with an amino acid in position 309 (HPO64);
in SEQ ID NO:32, beginning with an amino acid in position −35 and ending with an amino acid in position 129 (HPO70);
in SEQ ID NO:34, beginning with an amino acid in position −19 and ending with an amino acid in position 153 (HPO71);
in SEQ ID NO:36, beginning with an amino acid in position −25 and ending with an amino acid in position 176 (HPO76);
in SEQ ID NO:38, beginning with an amino acid in position −21 and ending with an amino acid in position 156 (HPO7);
in SEQ ID NO:40, beginning with an amino acid in position 1 and ending with an amino acid in position 144 (HPO80);
in SEQ ID NO:42, beginning with an amino acid in position −20 and ending with an amino acid in position 152 (HPO87);
in SEQ ID NO:44, beginning with an amino acid in position −31 and ending with an amino acid in position 112 (HPO95);
in SEQ ID NO:46, beginning with an amino acid in position −20 and ending with an amino acid in position 91 (HPO98);
in SEQ ID NO:48, beginning with an amino acid in position −21 and ending with an amino acid in position 129 (HPO9);
and
(b) the amino acid sequences encoded by the DNA inserts found in American Type Culture Collection deposit numbers 98197 (HPO76), 98210 (HPO18), 98201 (HPO121), 98208 (HPO45), 98198 (HPO101), 98200 (HPO116), 98211 (HPO7), 98199 (HPO104), 98214 (HPO15), 98206 (HPO58), 98202 (HPO132), 98203 (HPO9), 98204 (HPO38), 98205 (HPO87), 98217 (HPO71), 98219 (HPO70), 98215 (HPO80), 98216 (HPO95), 98218 (HPO98), 98220 (HPO57), 98207 (HPO50), 98213 (HPO64), 98212 (HPO54), and 98209 (HPO42);
or (ii) a derivative of said polypeptide.

25 more elements

A compound, in a substantially purified form, that is the mature form or a derivative of a polypeptide comprising an amino acid sequence that is homologous to an amino acid sequence of a polypeptide associated with the Helicobacter membrane, which is selected from the group consisting of:

(a) the amino acid sequences as shown:
in SEQ ID NO:2, beginning with an amino acid in position −27 and ending with an amino acid in position 160 (HPO101);
in SEQ ID NO:4, beginning with an amino acid in position 1 and ending with an amino acid in position 172 (HPO104);
in SEQ ID NO:6, beginning with an amino acid in position −17 and ending with an amino acid in position 169 (HPO116);
in SEQ ID NO:8, beginning with an amino acid in position −21 and ending with an amino acid in position 198 (HPO121);
in SEQ ID NO:10, beginning with an amino acid in position −20, and ending with an amino acid in position 132 (HPO132);
in SEQ ID NO:12, beginning with an amino acid in position 1 and ending with an amino acid in position 114 (HPO15);
in SEQ ID NO:14, beginning with an amino acid in position −17 and ending with an amino acid in position 248 (HPO18);
in SEQ ID NO:16, beginning with an amino acid in position −40 and ending with an amino acid in position 74 (HPO38);
in SEQ ID NO:18, beginning with an amino acid in position −31 and ending with an amino acid in position 226 (HPO42);
in SEQ ID NO:20, beginning with an amino acid in position −21 and ending with an amino acid in position 179 (HPO45);
in SEQ ID NO:22, beginning with an amino acid in position −33 and ending with an amino acid in position 114 (HPO50);
in SEQ ID NO:24, beginning with an amino acid in position −60 and ending with an amino acid in position 349 (HPO54);
in SEQ ID NO:26, beginning with an amino acid in position −18 and ending with an amino acid in position 288 (HPO57);
in SEQ ID NO:28, beginning with an amino acid in position −21 and ending with an amino acid in position 150 (HPO58);
in SEQ ID NO:30, beginning with an amino acid in position −20 and ending with an amino acid in position 309 (HPO64);
in SEQ ID NO:32, beginning with an amino acid in position −35 and ending with an amino acid in position 129 (HPO70);
in SEQ ID NO:34, beginning with an amino acid in position −19 and ending with an amino acid in position 153 (HPO71);
in SEQ ID NO:36, beginning with an amino acid in position −25 and ending with an amino acid in position 176 (HPO76);
in SEQ ID NO:38, beginning with an amino acid in position −21 and ending with an amino acid in position 156 (HPO7);
in SEQ ID NO:40, beginning with an amino acid in position 1 and ending with an amino acid in position 144 (HPO80);
in SEQ ID NO:42, beginning with an amino acid in position −20 and ending with an amino acid in position 152 (HPO87);
in SEQ ID NO:44, beginning with an amino acid in position −31 and ending with an amino acid in position 112 (HPO95);
in SEQ ID NO:46, beginning with an amino acid in position −20 and ending with an amino acid in position 91 (HPO98);
in SEQ ID NO:48, beginning with an amino acid in position −21 and ending with an amino acid in position 129 (HPO9);
and
(b) the amino acid sequences encoded by the Helicobacter DNA inserts found in American Type Culture Collection deposit numbers 98197 (HPO76), 98210 (HPO18), 98201 (HPO121), 98208 (HPO45), 98198 (HPO101), 98200 (HPO116), 98211 (HPO7), 98199 (HPO104), 98214 (HPO15), 98206 (HPO58), 98202 (HPO132), 98203 (HPO9), 98204 (HPO38), 98205 (HPO87), 98217 (HPO71), 98219 (HPO70), 98215 (HPO80), 98216 (HPO95), 98218 (HPO98), 98220 (HPO57), 98207 (HPO50), 98213 (HPO64), 98212 (HPO54), and 98209 (HPO42).

24 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/013,315Filed 5 Nov 2001
Priority: 14 Nov 1996
Published: 10 Apr 2003
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

C07K 16/121
Gram-negative bacteria: helicobacter ; Campylobacter
C07K 14/205
From bacteria: from Campylobacter
A61K 38/00
Medicinal preparations containing peptides
A61K 39/00
Medicinal preparations containing antigens or antibodies

Family

Application08/749,051Continuation · filed 14 Nov 1996
Application10/013,315Continuation · filed 5 Nov 2001
US 2003/0069404 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

The invention provides Helicobacter polypeptides that can be used in vaccination methods for preventing or treating Helicobacter infection, and polynucleotides that encode these polypeptides.

(a) the amino acid sequences as shown:
in SEQ ID NO:2, beginning with an amino acid in any one of the positions from −27 to 5, and ending with an amino acid in position 160 (HPO101);
in SEQ ID NO:4, beginning with an amino acid in position 1 and ending with an amino acid in position 172 (HPO104);
in SEQ ID NO:6, beginning with an amino acid in any one of the positions from −17 to 5, and ending with an amino acid in position 169 (HPO116);
in SEQ ID NO:8, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 198 (HPO121);
in SEQ ID NO:10, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 132 (HPO132);
in SEQ ID NO:12, beginning with an amino acid in positions 1 and ending with an amino acid in position 114 (HPO15);
in SEQ ID NO:14, beginning with an amino acid in any one of the positions from −17 to 5, and ending with an amino acid in position 248 (HPO18);
in SEQ ID NO:16, beginning with an amino acid in any one of the positions from −40 to 5, and ending with an amino acid in position 74 (HPO38);
in SEQ ID NO:18, beginning with an amino acid in any one of the positions from −34 to 5, and ending with an amino acid in position 226 (HPO42);
in SEQ ID NO:20, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 179 (HPO45);
in SEQ ID NO:22, beginning with an amino acid in any one of the positions from −33 to 5, and ending with an amino acid in position 114 (HPO50);
in SEQ ID NO:24, beginning with an amino acid in any one of the positions from −60 to 5, and ending with an amino acid in position 349 (HPO54);
in SEQ ID NO:26, beginning with an amino acid in any one of the positions from −18 to 5, and ending with an amino acid in position 288 (HPO57);
in SEQ ID NO:28, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 150 (HPO58);
in SEQ ID NO:30, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 309 (HPO64);
in SEQ ID NO:32, beginning with an amino acid in any one of the positions from −35 to 5, and ending with an amino acid in position 129 (HPO70);
in SEQ ID NO:34, beginning with an amino acid in any one of the positions from −19 to 5, and ending with an amino acid in position 153 (HPO71);
in SEQ ID NO:36, beginning with an amino acid in any one of the positions from −25 to 5, and ending with an amino acid in position 176 (HPO76);
in SEQ ID NO:38, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 156 (HPO7);
in SEQ ID NO:40, beginning with an amino acid in position 1 and ending with an amino acid in position 144 (HPO80);
in SEQ ID NO:42, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 152 (HPO87);
in SEQ ID NO:44, beginning with an amino acid in any one of the positions from −31 to 5, and ending with an amino acid in position 112 (HPO95);
in SEQ ID NO:46, beginning with an amino acid in any one of the positions from −20 to 5, and ending with an amino acid in position 91 (HPO98);
in SEQ ID NO:48, beginning with an amino acid in any one of the positions from −21 to 5, and ending with an amino acid in position 129 (HPO9);
and
(b) the precursor or mature amino acid sequences encoded by the Helicobacter DNA inserts found in American Type Culture Collection deposit numbers 98197 (HPO76), 98210 (HPO18), 98201 (HPO121), 98208 (HPO45), 98198 (HPO101), 98200 (HPO116), 98211 (HPO7), 98199 (HPO104), 98214 (HPO15), 98206 (HPO58), 98202 (HPO132), 98203 (HPO9), 98204 (HPO38), 98205 (HPO87), 98217 (HPO71), 98219 (HPO70), 98215 (HPO80), 98216 (HPO95), 98218 (HPO98), 98220 (HPO57), 98207 (HPO50), 98213 (HPO64), 98212 (HPO54), and 98209 (HPO42);
or (ii) a derivative of said polypeptide encoded by said polynucleotide.

25 more elements

Facts

Application

10/013,315Filed 5 Nov 2001

Priority

14 Nov 1996

Published

10 Apr 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C07K 16/121
Gram-negative bacteria: helicobacter ; Campylobacter
C07K 14/205
From bacteria: from Campylobacter
A61K 38/00
Medicinal preparations containing peptides
A61K 39/00
Medicinal preparations containing antigens or antibodies