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US 2003/0068786 A1Published application
Process for the production of egg yolk antibodies for organochlorine pesticides
OwnerBangalore Eahwar Amita Rani, Akmal Pasha +3inventors
InventorsBangalore Eahwar Amita Rani, Akmal Pasha, Nitturu Gopalakrishna Karanth, Raja Rao Jagannath Rao, Putte Gowda
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Abstract
The present invention provides relates to a process to produce egg yolk antibodies binding to small molecule organochlorine pesticides, by periodic immunization of the poultry birds with a desired hapten-protein conjugate in the breast muscle. Antibodies are harvested from egg yolk after five weeks interval.
Claims15
A process for the production of egg yolk antibodies binding to small molecule organo chlorine pesticides, the said process comprising the steps of:
- (a) selecting suitable from poultry birds;
- (b) immunizing the poultry birds with known complete adjuvant, each ml of said adjuvant comprising heat killed and dried 1 mg of Mycobacterium tuberculosis (H37Ra, ATCC 25177), 0.85 ml paraffin and 0.15 ml mannide monooleate;
- (c) immunizing the birds with 1000 μg conjugate selected from DDT-OH hapten, Octachloro cyclic hapten, 2,4,5 trichlorophoxyacetic acid β-alanine in breast muscle;
- (d) immunizing the birds again with the hapten-protein conjugate as given in step (c) with 500 μg of desired hapten conjugate;
- (e) immunizing the birds with hapten-protein conjugate at the intervals of two, three and five weeks;
- (f) immunizing the birds thereafter with hapten-protein conjugate at five weeks intervals as long as the bird lays eggs;
- (g) harvesting antibodies from the egg yolk of the birds.
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Dependencies are inferred from the claim text.