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US 2003/0068643 A1Published application
Methods and compositions for economically synthesizing and assembling long DNA sequences
OwnerThomas M. Brennan, Herbert L. Heynekerinventors
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Abstract
The present invention relates to a method for synthesizing and assembling long DNA sequences from short synthetic oligonucleotides. More specifically, the present invention is a cost-effective method for producing large segments of DNA of interest.
Claims17
A method for producing a biologically functional DNA sequence of greater than 200 bases long comprising the steps of:
- (a) synthesizing on a substrate an array of overlapping oligonucleotides from 10 to 200 bases encoding for either sense or antisense strand of said biologically functional DNA sequence wherein said oligonucleotides are covalently attached to the substrate using a cleavable linker;
- (b) cleaving said oligonucleotides from the substrate;
- and
- (c) assembling the mixture of overlapping oligonucleotides into said biologically functional DNA sequence.
1 more element
A method for optimizing the function of a DNA sequence comprising the steps of:
- (a) synthesizing on a substrate an array of overlapping oligonucleotides from 10-200 bases encoding for either sense or antisense strand of said DNA sequence wherein said oligonucleotides are covalently attached to the substrate using a cleavable linker;
- (b) cleaving said oligonucleotides from the substrate;
- (c) assembling the mixture of oligonucleotides into said DNA sequence;
- (d) testing the function of said DNA sequence;
- and
- (e) repeating the steps of (a)-(d) by varying said DNA sequence to optimize the function.
3 more elements
Dependencies are inferred from the claim text.