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AssigneeNanosphere, Inc.
InventorsWilliam Cork, Tim Patno, Mark Weber, Dave Morrow, Wesley Buckingham
Estimating the term…
An apparatus and method for imaging metallic nanoparticles is provided. Preferably, the invention provides for an apparatus and method for detection of gold colloid particles and for accurate reporting to the operator. The apparatus includes a substrate holder for holding the substrate, a processor and memory device, an imaging module, an illumination module, a power module, an input module, and an output module. The apparatus may have a stationary substrate holder and imaging module which are proximate to one another. The apparatus provided for a compact sized system without the need for complex motorized devices to move the camera across the substrate. Further, the apparatus and method provide for automatic detection of the spots/wells on the substrate, automatic quantification of the spots on the substrate, and automatic interpretation of the spots based on decision statistics.
Apparatus for detecting metallic nanoparticles on a substrate, with or without chemical signal amplification of the metallic nanoparticles, the apparatus comprising in combination:
In a substrate having a plurality of spots containing specific binding complements to one or more target analytes, at least one of the spots is a test spot for metallic nanoparticles complexed thereto in the presence of one or more target analytes, another spot is a control spot or a second test spot for metallic nanoparticles, with or without signal amplification, complexed thereto in the presence of a second or more target analytes, a method for detecting the presence or absence of the one or more of the target analytes in the test spot, the method comprising the steps of:
In a substrate having a plurality of spots containing specific binding complements to one or more target analytes, at least one of the spots is a test spot for metallic nanoparticles, with or without signal amplification, complexed thereto in the presence of one or more target analytes, another spot is a control spot or a second test spot for metallic nanoparticles complexed thereto in the presence of a second or more target analytes, an automatic method of detecting the plurality of spots comprising the steps of:
In a substrate having a plurality of wells, the wells containing at least two spots containing specific binding complements to one or more target analytes, at least one of the spots including a test spot having metallic nanoparticles, with or without signal amplification, complexed thereto in the presence of one or more target analytes, an automatic method of detecting the plurality of wells comprising the steps of:
Dependencies are inferred from the claim text.