Polypeptides that bind HIV gp120 and related nucleic acids, antibodies, compositions, and methods of use
AssigneeDepartment of Health and Human Services
InventorCarl Saxinger
Life of this patent
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Abstract
The present invention provides, among other things, a polypeptide that binds with the gp120 envelope protein of HIV, in particular HIV-1, under physiological conditions, a nucleic acid that encodes such a polypeptide and can be expressed in a cell, a composition comprising such a polypeptide or nucleic acid or an antibody and a carrier therefor, a composition comprising a solid support matrix to which is attached an above-described polypeptide or an anti-antibody to a specified polypeptide sequence, a method of making an antibody to gp120, and a method of removing HIV from a bodily fluid.
Claims55
A polypeptide comprising the amino acid sequence YDIXYYXXE, wherein X is any synthetic or naturally occurring amino acid residue, such that the polypeptide binds HIV gp120 under physiological conditions, and wherein said polypeptide comprises less than about 100 contiguous amino acids that are identical to or substantially identical to the amino acid sequence of the human CCR5 chemokine receptor.
A polypeptide comprising the amino acid sequence XEXIXIYXXXNYXXX, wherein X is any synthetic or naturally occurring amino acid, such that the polypeptide binds HIV gp120 under physiological conditions, and wherein said polypeptide less than about 100 contiguous amino acids that are identical to or substantially identical to the amino acid sequence of the human CXCR4 chemokine receptor.
A polypeptide comprising the amino acid sequence EHQAFLQFS, such that the polypeptide binds with HIV gp120 under physiological conditions and wherein said polypeptide comprises less than about 100 contiguous amino acids that are identical to or substantially identical to the amino acid sequence of the human STRL33 chemokine receptor.
A polypeptide comprising at least a portion or all of an amino acid sequence selected from the group consisting of LPPLYSLVFIFGFVGNML, QWDFGNTMCQLLTGLYFIGFFS, SQYQFWKNFQTLKIVILG, APYNIVLLLNTFQEFFGLNNCS, and YAFVGEKFRNYLLVFFQK, wherein the polypeptide binds with HIV gp120 under physiological conditions and comprises less than about 100 amino acid residues that are identical to or substantially identical to the amino acid sequence of the human CCR5 chemokine receptor.
A polypeptide comprising at least a portion or all of an amino acid sequence selected from the group consisting of LLLTIPDFIFANVSEADD (165-182), VVFQFQHIMVGLILPGIV (197-214), and IDSFILLEIIKQGCEFEN (261-278), wherein the polypeptide binds with HIV gp120 under physiological conditions and comprises less than about 100 amino acid residues that are identical to or substantially identical to the amino acid sequence of the human CXCR4 chemokine receptor.
A polypeptide comprising at least a portion or all of an amino acid sequence selected from the group consisting of LVISIFYHKLQSLTDVFL (53-70), PFWAYAGIHEWVFGQVMC (85-102), EAISTVVLATQMTLGFFL (185-202), LTMIVCYSVIIKTLLHAG (205-222), MAVFLLTQMPFNLMKFIRSTHW (237-258), HWEYYAMTSFHYTIMVTE (257-274), ACLNPVLYAFVSLKFRKN (281-298) and SKTFSASHNVEATSMFQL (325-342), wherein the polypeptide binds with-HIV gp120 under physiological conditions and comprises less than about 100 amino acid residues that are identical to or substantially identical to the amino acid sequence of the human STRL33 chemokine receptor.
A polypeptide comprising at least a portion of or all of an amino acid sequence selected from the group consisting of DTYICEVED, EEVQLLVFGLTANSD, THLLQGQSLTLTLES, and GEQVEFSFPLAFTVE, wherein the polypeptide binds with HIV gp120 under physiological conditions and wherein the polypeptide comprises less than about 100 amino acids that are identical to or substantially identical to the amino acid sequence of the human CD4 cell-surface protein.
A method of removing HIV from a bodily fluid of a mammal, which method comprises extra-corporeally contacting said bodily fluid with a solid support to which is attached a polypeptide of any of claims 1-28 or an anti-antibody to a polypeptide of any of claims 1-78, or the antibody of claim 54.
Dependencies are inferred from the claim text.