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US 2002/0177139 A1Published application
Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
AssigneeApplera Corporation
InventorsLawrence Greenfield, Luz Montesclaros
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Abstract
The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.
Claims64
A method for obtaining nucleic acid from a biological sample and binding the nucleic acid to a solid phase, comprising:
- contacting the biological sample with a disrupting buffer, wherein the disrupting buffer comprises:
- a protease;
- and
- a cationic surfactant;
- substantially neutralizing the cationic surfactant;
- and
- binding the nucleic acid to a solid phase.
4 more elements
A method for obtaining nucleic acid from a biological sample and binding the nucleic acid to a solid phase, comprising:
- contacting the biological sample with a disrupting buffer, wherein the disrupting buffer comprises:
- a protease;
- and
- a cationic surfactant;
- and
- binding the nucleic acid to a solid phase.
3 more elements
A kit comprising:
- a protease;
- a cationic surfactant;
- and
- a second surfactant, wherein the second surfactant substantially neutralizes the cationic surfactant.
1 more element
A kit for obtaining nucleic acid from a biological sample comprising:
- a protease;
- a cationic surfactant;
- a non-ionic surfactant, wherein the non-ionic surfactant permits the binding of nucleic acid to a solid phase in the presence of the protease and cationic surfactant;
- and
- a buffer with a high salt concentration.
2 more elements
Dependencies are inferred from the claim text.