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OwnerDaniel Scherman, Michael Bettan +1inventors
InventorsDaniel Scherman, Michael Bettan, Pascal Bigey
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The present invention relates to novel constructs and compositions and to a novel method for regulating the expression of a transgene of interest in vivo by conditional inhibition, and to the uses thereof in experimental, clinical and therapeutic domains or for the production of animals or plants. For example, the novel regulation method is based on the coexpression of a transgene of interest encoding a transcript of interest and of an inhibitory transgene encoding an inhibitory transcript specific for the transcript of interest, so as to obtain constitutive inhibition of the activity of the transcript of interest, and to be able to ensure effective regulation of the transcript of interest, either by inhibiting its inhibitory transcript, or by activating the transcript of interest, or alternatively by activating the transcript of interest and concomitantly inhibiting its inhibitory transcript.
A method for regulating the expression of a transgene of interest in vivo comprising:
A composition comprising a first nucleic acid comprising a sequence of a transgene of interest encoding a transcript of interest, and comprising a second nucleic acid comprising a sequence of an inhibitory transgene encoding an inhibitory transcript specific for said transcript of interest, wherein the sequences are each under the control of a transcriptional promoter, and wherein the activity of the inhibitory transcript is optionally regulated with at least one external agent and the activity of the transcript of interest is optionally regulated with at least one external agent.
64 The composition according to claim 46, wherein the transgene of interest encodes an activator which is involved in the expression of another gene.
A transgenic animal, wherein the animal carries a first nucleic acid comprising the sequence of a transgene of interest encoding a transcript of interest, and a second nucleic acid comprising the sequence of an inhibitory transgene encoding an inhibitory transcript specific for the transcript of interest, wherein each of the sequences are under the control of a transcriptional promoter, and the activity of the inhibitory transcript is optionally regulated with at least one external agent, and the activity of the transcript of interest is optionally regulated with at least one external agent.
A transgenic plant, wherein the plant carries a first nucleic acid comprising the sequence of a transgene of interest encoding a transcript of interest, and a second nucleic acid comprising the sequence of an inhibitory transgene encoding an inhibitory transcript specific for said transcript of interest, wherein each of the sequences are under the control of separate transcriptional promoters, and the activity of the inhibitory transcript is optionally regulated with at least one external agent, and the activity of the transcript of interest is optionally regulated with at least one external agent.
Dependencies are inferred from the claim text.